Salt acclimation in sorghum plants by exogenous proline: physiological and biochemical changes and regulation of proline metabolism
| Autor: | José Tarquinio Prisco, Daniel Gomes Coelho, Enéas Gomes-Filho, Rafael de Souza Miranda, Francisco Dalton Barreto de Oliveira, Paulo André Ferreira de Freitas, José Hélio Costa, Humberto Henrique de Carvalho, Kátia Daniella da Cruz Saraiva |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Proline Plant Science Photosynthesis 01 natural sciences Acclimatization 03 medical and health sciences Proline dehydrogenase activity Sorghum biology Sodium food and beverages General Medicine Metabolism biology.organism_classification Salinity 030104 developmental biology Biochemistry Potassium Osmoprotectant Calcium Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant cell reports. 38(3) |
| ISSN: | 1432-203X |
| Popis: | Mitigation of deleterious effects of salinity promoted by exogenous proline can be partially explained by changes in proline enzymatic metabolism and expression of specific proline-related genes. Proline accumulation is a usual response to salinity. We studied the ability of exogenous proline to mitigate the salt harmful effects in sorghum (Sorghum bicolor) leaves. Ten-day-old plants were cultivated in Hoagland’s nutrient solution in either the absence or presence of salinity (NaCl at 75 mM) and sprayed with distilled water or 30 mM proline solution. Salinity deleterious effects were alleviated by exogenous proline 14 days after treatment, with a return in growth and recovery of leaf area and photosynthetic parameters. Part of the salinity response reflected an improvement in ionic homeostasis, provided by reduction in Na+ and Cl− ions and increases in K+ and Ca2+ ions as well as increases of compatible solutes. In addition, the application of proline decreased membrane damage and did not increase relative water content. Proline-treated salt-stressed plants displayed increase in proline content, a response counterbalanced by punctual modulation in proline synthesis (down-regulation of Δ1-pyrroline-5-carboxylate synthetase activity) and degradation (up-regulation of proline dehydrogenase activity) enzymes. These responses were correlated with expression of specific proline-related genes (p5cs1 and prodh). Our findings clearly show that proline treatment results in favorable changes, reducing salt-induced damage and improving salt acclimation in sorghum plants. |
| Databáze: | OpenAIRE |
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