High-performance liquid chromatographic assay for amiloride in plasma and urine
| Autor: | Ji-Hong Zhou, Xiao-Quan Liu, Dan-Ke Xu, Sheng-Kai Huang, Yi-Sheng Yuan |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Detection limit
Chromatography Calibration curve Administration Oral Biological Availability General Chemistry Urine High-performance liquid chromatography Fluorescence spectroscopy Amiloride chemistry.chemical_compound Spectrometry Fluorescence chemistry Injections Intravenous medicine Animals Humans Protein precipitation Indicators and Reagents Rabbits Perchloric acid Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 567:451-458 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(91)80151-2 |
| Popis: | A sensitive and simplified high-performance liquid chromatographic procedure has been developed for quantification of amiloride in rabbit plasma, as well as human plasma and urine. Following protein precipitation with perchloric acid, the supernatant was directly injected into a C18 Nucleosil column. The mobile phase consisted of methanol-water (45:55) containing 0.1 M perchloric acid, and the compound was quantitated using a fluorescence detector at excitation and emission wavelengths of 286 and 418 nm, respectively. The average recovery was 97.6%. The calibration curve was linear over the range 2.0-20.0 ng/ml. The limit of detection was 0.5 ng/ml. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|