eDNA for detection of five highly invasive molluscs. A case study in urban rivers from the Iberian Peninsula

Autor: Carmelo Escot, Laura Miralles, Eva Garcia-Vazquez, Ana Basanta, Laura Clusa
Rok vydání: 2017
Předmět:
0106 biological sciences
Biodiversity
Invasive Species
Marine and Aquatic Sciences
Artificial Gene Amplification and Extension
Introduced species
Polymerase Chain Reaction
01 natural sciences
Invasive species
Aquaculture
RNA
Ribosomal
16S
DNA extraction
Geographic Areas
Multidisciplinary
Geography
biology
Ecology
Eukaryota
Medicine
Research Article
Freshwater Environments
Urban Areas
Science
Research and Analysis Methods
Mytilopsis leucophaeata
010603 evolutionary biology
Electron Transport Complex IV
Extraction techniques
Rivers
Species Colonization
Surface Water
Animals
14. Life underwater
Corbicula fluminea
Molecular Biology Techniques
Molecular Biology
business.industry
010604 marine biology & hydrobiology
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Aquatic Environments
DNA
Molluscs
Bodies of Water
biology.organism_classification
Sinanodonta woodiana
Melanoides
Invertebrates
Fishery
Lakes
Mollusca
Spain
Earth Sciences
Hydrology
Introduced Species
business
Zdroj: Scopus
RUO. Repositorio Institucional de la Universidad de Oviedo
instname
PLOS ONE
PLoS ONE, Vol 12, Iss 11, p e0188126 (2017)
PLoS ONE
Popis: Biological invasions are an important threat to biodiversity especially in aquatic ecosystems, and their frequency is generally higher near urban areas. Potentially invasive non-indigenous molluscs were deliberately introduced into European waters for food (Corbicula fluminea) and biocontrol (Melanoides tuberculata), and unintentionally introduced by ballast water (Mytilopsis leucophaeata, Corbicula fluminea), stock contamination (Sinanodonta woodiana), accidental escapes from aquaculture (Sinanodonta woodiana), aquarium trade releases (Melanoides tuberculata) and even attached to aquatic birds (Corbicula fluminea). Three rivers from the Iberian Peninsula were monitored near the three most populated inland cities to evaluate the presence of these invasive molluscs through PCR amplification using taxon-specific primers from eDNA. New primers were designed within 16S rRNA and cytochrome oxidase subunit I genes, tested in silico from BLAST methodology and experimentally in vitro before application in the field. C. fluminea was found in Ebro River (near Zaragoza); M. leucophaeata in Guadalquivir River (near Sevilla). M. tuberculata and S. woodiana were found from enclosed areas (lake and reservoir respectively) upstream, respectively, Zaragoza and Madrid. The new tools are ready to be used in other regions where these species are also invasive.
Databáze: OpenAIRE
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