Pre-Injection of Small Interfering RNA (siRNA) Promotes c-Jun Gene Silencing and Decreases the Survival Rate of Axotomy-Injured Spinal Motoneurons in Adult Mice
| Autor: | Ying-qin Li, Fa-huan Song, Ke Zhong, Guang-yin Yu, Prince Last Mudenda Zilundu, Ying-ying Zhou, Rao Fu, Ying Tang, Ze-min Ling, Xiaoying Xu, Li-hua Zhou |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Small interfering RNA Accessory Nerve Injuries medicine.medical_treatment Biology Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine medicine Animals Spinal Cord Ventral Horn Brachial Plexus Gene Silencing Motor Neurons Mice Inbred BALB C Plexus JNK Mitogen-Activated Protein Kinases General Medicine medicine.disease Spinal cord Cell biology RNAi Therapeutics 030104 developmental biology medicine.anatomical_structure nervous system Brachial plexus injury Axotomy Brachial plexus 030217 neurology & neurosurgery Reinnervation |
| Zdroj: | Journal of Molecular Neuroscience. 65:400-410 |
| ISSN: | 1559-1166 0895-8696 |
| DOI: | 10.1007/s12031-018-1098-y |
| Popis: | Brachial plexus injury is a common clinical peripheral nerve trauma. A series of genes in motoneurons were activated in the corresponding segments of the spinal cord after brachial plexus roots axotomy. The spatial and temporal expression of these genes directly affects the speed of motoneuron axon regeneration and precise target organ reinnervation. In a previous study, we observed the overexpression of c-Jun in motoneurons of the spinal cord ventral horn after brachial plexus injury in rats. However, the relevance of c-Jun expression with respect to the fate of axotomy-induced branchial plexus injury in adult mice remains unknown. In the present study, we explored the function of c-Jun in motoneuron recovery after axotomy. We pre-injected small interfering RNA (siRNA) to knockdown c-Jun expression in mice and examined the effects of the overexpression of c-Jun in motoneurons after the axotomy of the brachial plexus in vivo. Axotomy induced c-Jun overexpression in the ventral horn motoneurons of adult mice from 3 to 14 days after injury. In addition, the pre-injection of siRNA transiently inhibited c-Jun expression and decreased the survival rate of axotomy-injured motoneurons. These findings indicate that the axotomy-induced overexpression of c-Jun plays an important role in the survival of ventral horn motoneurons in adult mice. In addition, the pre-injection of c-Jun siRNA through the brachial plexus stem effectively adjusts c-Jun gene expression at the ipsilateral side. |
| Databáze: | OpenAIRE |
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