The Health Promoting Effects of the Fruiting Bodies Extract of the Peppery Milk Cap Mushroom Lactarius piperatus (Agaricomycetes) from Serbia
| Autor: | Nevena Petrović, Olivera Milošević-Djordjević, Jovana Tubic, Darko Grujičić, Marijana Kosanić, Tatjana Stanojković, Aleksandra Marković |
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| Rok vydání: | 2020 |
| Předmět: |
Cell Extracts
Antioxidant Cell Survival DPPH medicine.medical_treatment Antineoplastic Agents Microbial Sensitivity Tests 010501 environmental sciences Protective Agents 01 natural sciences Applied Microbiology and Biotechnology Inhibitory Concentration 50 chemistry.chemical_compound Anti-Infective Agents Phenols Cell Line Tumor Lactarius Drug Discovery medicine Humans Fruiting Bodies Fungal Food science 0105 earth and related environmental sciences 2. Zero hunger Pharmacology Mushroom biology 010405 organic chemistry Superoxide Basidiomycota biology.organism_classification Antimicrobial 0104 chemical sciences Edible mushroom Comet assay chemistry Serbia |
| Zdroj: | International Journal of Medicinal Mushrooms. 22:347-357 |
| ISSN: | 1521-9437 |
| Popis: | Antioxidant, antimicrobial, genoprotective, anticancer, and neuroprotective potential of acetone extract of the fruiting bodies of the edible mushroom Lactarius piperatus was studied. The antioxidant activity was evaluated using different methods (DPPH radical scavenging, superoxide anion radicals scavenging, reducing power assay, and determination of total phenolic compounds). The microdilution method was used to reveal the antimicrobial potential. The genoprotective potential was determined by Comet assay. Cytotoxic activity was tested using MTT. The capacity of the extract to inhibit acetylcholinesterase was used for determining its neuroprotective potential. The received results show that L. piperatus extract possessed potent health enhancing effects. In the antioxidant activity, IC50 was 33.97 μg/mL for DPPH radicals scavenging and 22.52 μg/mL for superoxide anion radicals scavenging, whereas the absorbance for the reducing power was from 0.0510 to 0.1451. The total content of phenolic compounds in the extract was 5.08 μg PE/mg. The testing of the antimicrobial activity showed that MIC values were from 0.039 to 10 mg/mL. For Comet assay, all concentrations of extract increased the GDI values from 0.46 ± 0.05 to 0.99 ± 0.31. L. piperatus extract expressed relatively strong cytotoxic activity with IC50 values ranging from 37.83 to 65.94 μg/mL. Finally, the percentage of inhibition of acetylcholinesterase activity of tested extract was within the range 16.75-44.35%. Our results imply that the acetone extract of L. piperatus has rather strong antioxidant, antimicrobial, genoprotective, anticancer, and neuroprotective effects; thus this mushroom represents healthy food that could be used in the pharmaceutical industry and to prevent various diseases. |
| Databáze: | OpenAIRE |
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