Vertical advection of extracellular DNA by water capillarity in soil columns
| Autor: | Paolo Nannipieri, Maria Teresa Ceccherini, Timothy M. Vogel, Giacomo Pietramellara, Judith Ascher |
|---|---|
| Přispěvatelé: | Dipartimento Scienza del Suolo e Nutrizione della Pianta, Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), Ampère (AMPERE), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Capillary fringe
Capillary action Soil Science Microbiology law.invention Nucleic acid movement 03 medical and health sciences chemistry.chemical_compound Water-capillary rise law Extracellular DNA Polymerase chain reaction PCR monitoring 0303 health sciences Chromatography 030306 microbiology [SPI.NRJ]Engineering Sciences [physics]/Electric power 04 agricultural and veterinary sciences 6. Clean water chemistry Biochemistry Soil water 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Soil microcosm Wetting Microcosm DNA Groundwater |
| Zdroj: | Soil Biology and Biochemistry Soil Biology and Biochemistry, Elsevier, 2007, 39 (1), pp.158-163. ⟨10.1016/j.soilbio.2006.07.006⟩ |
| ISSN: | 0038-0717 |
| DOI: | 10.1016/j.soilbio.2006.07.006⟩ |
| Popis: | The fate of extracellular DNA in the environment concerns both the fate of transgenes from genetically modified organisms and the evolution of active bacteria capable of incorporating this DNA into their genomes. This study addressed the possibility that DNA, like other organic molecules, could move vertically in the capillary fringe of groundwater aquifers. The targeted gene fragment used here was the 35S-nptII sequence, which was below detection levels in controls. Initial microcosm studies detected the DNA target molecule by PCR during the entire experiment. The vertical advection of water and DNA were monitored for a period of 3 days in soil columns. DNA was added as a water solution at the bottom of the unsaturated soil column, and then DNA-free water was added at the bottom after 12 and 24 h. After the addition of the DNA solution, capillary water rose 4 cm within the soil column and the target DNA was detected up to that height. After 60 min, the entire soil column (10 cm) was wetted and the target sequence was detected up to a height of 7.5 cm. After the second wetting (12 h later), the target sequence was detected up to the top of the soil column (10 cm). However, after the third wetting (24 h later), the marker sequence was only found at heights from 0.5 to 4 cm. Results clearly show the vertical movement of DNA due the capillary rise and suggest the possibility of DNA degradation within the soil column. |
| Databáze: | OpenAIRE |
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