Reduced CENPU expression inhibits lung adenocarcinoma cell proliferation and migration through PI3K/AKT signaling

Autor: Ya-Yan Wang, Jun Li, Long-Bin Pang, Ronghua Zhang, Zhi-Guang Wang
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Lung Neoplasms
Adenocarcinoma of Lung
Cell Cycle Proteins
Biology
Applied Microbiology and Biotechnology
Biochemistry
Analytical Chemistry
Cell Line
Histones
03 medical and health sciences
Phosphatidylinositol 3-Kinases
0302 clinical medicine
Western blot
Downregulation and upregulation
Cell Movement
Cell Line
Tumor
Centromere
medicine
Humans
Neoplasm Invasiveness
Neoplasm Metastasis
Molecular Biology
Mitosis
Protein kinase B
PI3K/AKT/mTOR pathway
Cell Proliferation
medicine.diagnostic_test
Cell growth
Organic Chemistry
Nuclear Proteins
General Medicine
Middle Aged
medicine.disease
030104 developmental biology
030220 oncology & carcinogenesis
Gene Knockdown Techniques
Cancer research
bacteria
Adenocarcinoma
Female
Proto-Oncogene Proteins c-akt
Biotechnology
Signal Transduction
Zdroj: Bioscience, biotechnology, and biochemistry. 83(6)
ISSN: 1347-6947
Popis: CENPU (centromere protein U), a centromere component essential for mitosis, relates with some cancers progression. However, it is not well illustrated in lung adenocarcinoma (LAC). Here, we aimed to investigate the potential effect of CENPU on LAC progression and prognosis. In this experiment, expression level of CENPU and association between its expression and LAC patients’ clinicopathological characteristics and prognosis were analyzed. The proliferation, migration and invasive abilities of LAC cells were determined by CCK-8, colony formation, transwell assays. Western blot was used to detect PI3K/AKT signaling key proteins. We found CENPU level was overexpressed in LAC tissues on comparing normal tissues. Moreover, CENPU overexpression correlated with clinicopathological variables and predicted an independent prognostic indicator in LAC patients. Functionally, CENPU downregulation significantly inhibited LAC cell proliferation, migration and invasion in, which was possibly mediated by PI3K/AKT pathway inactivation. Our findings insinuate targeting CENPU may be a potential therapeutic strategy for LAC.
Databáze: OpenAIRE
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