Anti-Mycobacterium tuberculosis activity and cytotoxicity of Calophyllum brasiliense Cambess (Clusiaceae)
| Autor: | Rosilene Fressatti Cardoso, Claudia Terencio Agostinho Pires, Diógenes Aparício Garcia Cortez, Luciana Dias Ghiraldi Lopes, Regiane Bertin de Lima Scodro, Vera Lúcia Dias Siqueira, Mislaine Adriana Brenzan |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 lcsh:QR1-502 Microbial Sensitivity Tests Amentoflavone coumarin High-performance liquid chromatography lcsh:Microbiology Mycobacterium tuberculosis chemistry.chemical_compound Biflavonoids Calophyllum brasiliense Dichloromethane Methylene Chloride Chromatography biology Traditional medicine Plant Extracts Macrophages Mammea Clusiaceae Resazurin Articles biology.organism_classification Anti-Bacterial Agents chemistry Calophyllum cytotoxicity - 3-(4 5-dimethyl thiazol-2-yl)-2 5-diphenyl tetrazolium bromide |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 109, Iss 3, Pp 324-329 (2014) Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz, Volume: 109, Issue: 3, Pages: 324-329, Published: 04 MAR 2014 |
| ISSN: | 1678-8060 0074-0276 |
| DOI: | 10.1590/0074-0276130323 |
| Popis: | We evaluated the in vitro anti-Mycobacterium tuberculosis activity and the cytotoxicity of dichloromethane extract and pure compounds from the leaves of Calophyllum brasiliense. Purification of the dichloromethane extract yielded the pure compounds (-) mammea A/BB (1), (-) mammea B/BB (2) and amentoflavone (3). The compound structures were elucidated on the basis of spectroscopic and spectrometric data. The contents of bioactive compounds in the extracts were quantified using high performance liquid chromatography coupled to an ultraviolet detector. The anti-M. tuberculosis activity of the extracts and the pure compounds was evaluated using a resazurin microtitre assay plate. The cytotoxicity assay was performed in J774G.8 macrophages using the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colourimetric method. The quantification of the dichloromethane extract showed (1) and (2) at concentrations of 31.86 ± 2.6 and 8.24 ± 1.1 µg/mg of extract, respectively. The dichloromethane and aqueous extracts showed anti-M. tuberculosis H37Rv activity of 62.5 and 125 µg/mL, respectively. Coumarins (1) and (2) showed minimal inhibitory concentration ranges of 31.2 and 62.5 µg/mL against M. tuberculosis H37Rv and clinical isolates. Compound (3) showed no activity against M. tuberculosis H37Rv. The selectivity index ranged from 0.59-1.06. We report the activity of the extracts and coumarins from the leaves of C. brasiliense against M. tuberculosis. |
| Databáze: | OpenAIRE |
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