Raman spectroscopic analysis of the clonal and horizontal spread of CTX-M-15-producing Klebsiella pneumoniae in a neonatal intensive care unit
| Autor: | Y. Aujard, V. Biran, Lucien Brasme, Edouard Bingen, C. De Champs, W. van Leeuwen, Didier Moissenet, Kees Maquelin, C. Doit, Céline Courroux, Hoang Vu-Thien, Thomas Guillard, K. Guyot |
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| Přispěvatelé: | Dermatology, Medical Microbiology & Infectious Diseases |
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Male Neonatal intensive care unit Cefotaxime medicine.drug_class Klebsiella pneumoniae Cephalosporin Gestational Age Drug resistance Microbial Sensitivity Tests Biology Amoxicillin-Potassium Clavulanate Combination Spectrum Analysis Raman Polymerase Chain Reaction beta-Lactamases Microbiology Disease Outbreaks Plasmid Risk Factors Intensive care Drug Resistance Multiple Bacterial Intensive Care Units Neonatal medicine Disease Transmission Infectious Humans Typing Infant Newborn General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Virology Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Klebsiella Infections Infectious Diseases Genes Bacterial Fomites Female France medicine.drug Plasmids |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases, 31(10), 2827-2834. Springer-Verlag |
| ISSN: | 1435-4373 0934-9723 |
| Popis: | Nosocomial outbreaks of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae are an increasing concern in neonatal intensive care units (NICUs). We describe an outbreak of ESBL-producing K. pneumoniae that lasted 5 months and affected 23 neonates in our NICU. Proton pump inhibitor and extended-spectrum cephalosporin exposure were significantly associated with the risk of ESBL-producing K. pneumoniae colonisation and/or infection. Thirty isolates recovered from clinical, screening and environmental samples in the NICU were studied by means of Raman spectroscopy, pulsed-field gel electrophoresis and repetitive extragenic palindromic polymerase chain reaction (rep-PCR). The Raman clustering was in good agreement with the results of the other two molecular methods. Fourteen isolates belonged to the Raman clone 1 and 16 to the Raman clone 3. Molecular analysis showed that all the strains expressed SHV-1 chromosomal resistance, plasmid-encoded TEM-1 and CTX-M-15 beta-lactamases. Incompatibility groups of plasmid content identified by PCR-based replicon typing indicated that resistance dissemination was due to the clonal spread of K. pneumoniae and horizontal CTX-M-15 gene transfer between the two clones. |
| Databáze: | OpenAIRE |
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