Replication and virulence of pseudorabies virus mutants lacking glycoprotein gX
| Autor: | D R Thomsen, R J Yancey, C C Marchioli, Leonard E. Post |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1987 |
| Předmět: |
DNA Replication
Genes Viral viruses Immunology Mutant Virulence Pseudorabies medicine.disease_cause Virus Replication Microbiology Virus Viral Proteins Plasmid Viral Envelope Proteins Virology medicine Animals Gene Mutation biology virus diseases DNA Restriction Enzymes biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology Herpesvirus 1 Suid Viral replication Genes Insect Science Research Article Plasmids |
| Popis: | Pseudorabies virus (PRV) glycoprotein gX accumulates in the medium of infected cells. In an attempt to study the function of gX, two viruses were constructed that lacked a functional gX gene. One virus, PRV delta GX1, was derived by insertion of the herpes simplex virus thymidine kinase gene into the gX-coding region. The other virus, PRV delta GXTK-, was derived by subsequent deletion of the inserted herpes simplex virus thymidine kinase gene. Both viruses replicated in cell cultures but produced no gX. Furthermore, PRV delta GX1 was capable of killing mice with a 50% lethal dose of less than 100 PFU. |
| Databáze: | OpenAIRE |
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