| Autor: |
Giuseppe M. Paternò, Guglielmo Lanzani, Letizia Colella, Mattia Bramini, Simone Cimò, Luca Maragliano, Fabio Benfenati, Chiara Bertarelli, Daniele Fazzi, José Fernando Maya-Vetencourt, Cyril G. Eleftheriou, Mattia L. DiFrancesco, Francesco Lodola, Elisabetta Colombo |
| Rok vydání: |
2019 |
| Předmět: |
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| Popis: |
Optical technologies allowing modulation of neuronal activity at high spatio-temporal resolution are becoming paramount in neuroscience. We engineered novel light-sensitive molecules by adding polar groups to a hydrophobic backbone containing azobenzene and azepane moieties. We demonstrate that the probes stably partition into the plasma membrane, with affinity for lipid rafts, and cause thinning of the bilayer through their trans-dimerization in the dark. In neurons pulse-labeled with the compound, light induces a transient hyperpolarization followed by a delayed depolarization that triggers action potential firing. The fast hyperpolarization is attributable to a light-dependent decrease in capacitance due to membrane relaxation that follows disruption of the azobenzene dimers. The physiological effects are persistent and can be evoked in vivo after labeling the mouse somatosensory cortex. These data demonstrate the possibility to trigger neural activity in vitro and in vivo by modulating membrane capacitance, without directly affecting ion channels or local temperature. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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