Transplantation of a 3D-printed tracheal graft combined with iPS cell-derived MSCs and chondrocytes
| Autor: | Hana Cho, In Gul Kim, Shin Hyae Lee, Sang Jin Lee, Ji Suk Choi, Seong Keun Kwon, Su A Park, Yoo Wook Kwon |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Respiratory Mucosa Male Pathology medicine.medical_specialty Induced Pluripotent Stem Cells lcsh:Medicine 02 engineering and technology Article 03 medical and health sciences Chondrocytes In vivo medicine Animals Regeneration Induced pluripotent stem cell lcsh:Science Cells Cultured Multidisciplinary Tracheal Diseases Tissue Engineering Tissue Scaffolds Chemistry Cartilage Regeneration (biology) Mesenchymal stem cell lcsh:R Ciliated columnar epithelium Mesenchymal Stem Cells respiratory system 021001 nanoscience & nanotechnology Transplantation Trachea Experimental models of disease 030104 developmental biology medicine.anatomical_structure Printing Three-Dimensional lcsh:Q Rabbits 0210 nano-technology Biomedical engineering |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) |
| ISSN: | 2045-2322 |
| Popis: | For successful tracheal reconstruction, tissue-engineered artificial trachea should meet several requirements, such as biocompatible constructs comparable to natural trachea, coverage with ciliated respiratory mucosa, and adequate cartilage remodeling to support a cylindrical structure. Here, we designed an artificial trachea with mechanical properties similar to the native trachea that can enhance the regeneration of tracheal mucosa and cartilage through the optimal combination of a two-layered tubular scaffold and human induced pluripotent stem cell (iPSC)-derived cells. The framework of the artificial trachea was fabricated with electrospun polycaprolactone (PCL) nanofibers (inner) and 3D-printed PCL microfibers (outer). Also, human bronchial epithelial cells (hBECs), iPSC-derived mesenchymal stem cells (iPSC-MSCs), and iPSC-derived chondrocytes (iPSC-Chds) were used to maximize the regeneration of tracheal mucosa and cartilage in vivo. After 2 days of cultivation using a bioreactor system, tissue-engineered artificial tracheas were transplanted into a segmental trachea defect (1.5-cm length) rabbit model. Endoscopy did not reveal granulation ingrowth into tracheal lumen. Alcian blue staining clearly showed the formation of ciliated columnar epithelium in iPSC-MSC groups. In addition, micro-CT analysis showed that iPSC-Chd groups were effective in forming neocartilage at defect sites. Therefore, this study describes a promising approach for long-term functional reconstruction of a segmental tracheal defect. |
| Databáze: | OpenAIRE |
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