Voltage-dependent calcium channel subtypes controlling somatic substance P release in the peripheral nervous system
| Autor: | David J. Beadle, Bermudez Isabel, Louise M. Harding |
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| Rok vydání: | 2000 |
| Předmět: |
Male
medicine.medical_specialty Calcium Channels L-Type chemistry.chemical_element Substance P Tris-buffered saline Calcium In Vitro Techniques Rats Sprague-Dawley chemistry.chemical_compound Calcium Channels N-Type In vivo Internal medicine Ganglia Spinal Peripheral Nervous System medicine Animals Biological Psychiatry Pharmacology Neurons Voltage-dependent calcium channel Chemistry Depolarization Calcium Channels P-Type Calcium Channel Blockers Immunohistochemistry Rats Electrophysiology Microscopy Electron medicine.anatomical_structure Endocrinology Peripheral nervous system Potassium Female Calcium Channels Neurosecretion Ion Channel Gating |
| Zdroj: | Progress in neuro-psychopharmacologybiological psychiatry. 23(6) |
| ISSN: | 0278-5846 |
| Popis: | 1. Isolated rat dorsal root ganglia (DRG) neurones support vesicular, non synaptic release of substance P in a depolarisation and Ca2+ dependent manner. 2. In vivo this process may mediate cross-communication between DRG cells in some neuropathological conditions and is therefore a putative area for drug intervention. 3. The authors investigated the voltage-dependent Ca2+ channel (VDCC) subtypes involved in somatic release of substance P. Fresh (1 day) cultures of DRG neurones were incubated with high K+ depolarising saline in the presence and absence of subtype selective VDCC blockers. Substance P released into the external media was collected and quantified using a radioimmunoassay. 4. The results show that L-type and N-type, but not P-type, VDCCs play an important role in high K+ evoked substance P release from rat DRG neurones. |
| Databáze: | OpenAIRE |
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