Neurovirulence in Mice of H5N1 Influenza Virus Genotypes Isolated from Hong Kong Poultry in 2001
| Autor: | Malik Peiris, Jerold E. Rehg, Yi Guan, Daniel R. Perez, Aleksandr S. Lipatov, Scott Krauss, Robert G. Webster |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
viruses
Immunology Molecular Sequence Data Viral Plaque Assay Biology medicine.disease_cause Microbiology Virus Cell Line Poultry Diseases - virology Mice Goose Virology biology.animal Central Nervous System Viral Diseases - pathology - physiopathology - virology Genotype medicine Animals Amino Acid Sequence H5N1 virus Gene Poultry Diseases chemistry.chemical_classification Influenza in Birds - virology Influenza A Virus H5N1 Subtype Virulence Influenza A virus - chemistry - classification - genetics - pathogenicity virus diseases Brain Sequence Analysis DNA Pathogenicity Influenza A virus subtype H5N1 Amino acid chemistry Spinal Cord Influenza A virus Organ Specificity Insect Science Influenza in Birds Central Nervous System Viral Diseases Pathogenesis and Immunity Hong Kong Chickens |
| Popis: | We studied the pathogenicity of five different genotypes (A to E) of highly pathogenic avian H5N1 viruses, which contained HA genes similar to those of the H5N1 virus A/goose/Guangdong/1/96 and five different combinations of "internal" genes, in a mouse model. Highly pathogenic, neurotropic variants of genotypes A, C, D, and E were isolated from the brain after a single intranasal passage in mice. Genotype B virus was isolated from lungs only. The mouse brain variants had amino acid changes in all gene products except PB1, NP, and NS1 proteins but no common sets of mutations. We conclude that the original H5N1/01 isolates of genotypes A, C, D, and E were heterogeneous and that highly pathogenic neurotropic variants can be rapidly selected in mice. published_or_final_version |
| Databáze: | OpenAIRE |
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