Vitamin B6 suppresses serine protease inhibitor 3 expression in the colon of rats and in TNF-α-stimulated HT-29 cells
| Autor: | Norihisa Kato, Tomomi Ohata, Atsuko Hirata, Noriyuki Yanaka, Keigo Toya, Mayumi Kanda |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Vitamin Colon Vitamin D-binding protein Biology chemistry.chemical_compound NF-KappaB Inhibitor alpha Gene expression medicine Animals Anticarcinogenic Agents Humans RNA Messenger Rats Wistar Pyridoxal Serpins Oligonucleotide Array Sequence Analysis Tumor Necrosis Factor-alpha Ubiquitination Pyridoxine Molecular biology Recombinant Proteins Vitamin B 6 Protein ubiquitination Rats IκBα Gene Expression Regulation chemistry Colonic Neoplasms I-kappa B Proteins Tumor necrosis factor alpha Vitamin B 6 Deficiency HT29 Cells Acute-Phase Proteins Food Science Biotechnology medicine.drug |
| Zdroj: | Molecular Nutrition & Food Research. 55:635-643 |
| ISSN: | 1613-4125 |
| DOI: | 10.1002/mnfr.201000282 |
| Popis: | Scope: Previous reports in the areas of animal studies and, recently epidemiology, have linked anti-tumorigenic and anti-inflammatory effects to dietary vitamin B6. This study investigated the molecular mechanism of these effects of vitamin B6. Methods and results: DNA microarray analysis was used to obtain information on changes in colon gene expression from vitamin B6 (pyridoxine) repletion in vitamin B6-deficient rats. Pyridoxine supplementation down-regulated the inflammatory molecule, serine protease inhibitor clade A member 3 (SPI-3) mRNA expression in the colon. This study also showed that tumor necrosis factor α (TNF-α) induced SPI-3 mRNA expression in HT-29 human colon cancer cells, and vitamin B6 (pyridoxal hydrochloride) pretreatment of HT-29 cells inhibited TNF -induced mRNA expression of SPI-3. Vitamin B6 inhibited TNF-α-induced NF-κB activation via suppression of IκBα degradation in HT-29 cells. HT-29 cells stably expressing epitope-tagged ubiquitin were generated and vitamin B6 pretreatment was shown to inhibit ubiquitination of the IkB protein in response to TNF-α-i. Conclusion: Vitamin B6 suppressed SPI-3 expression in the colon of rats and in TNF-α-stimulated HT-29 cells. Further, this study showed a possible role of vitamin B6 in the regulation of protein ubiquitination. |
| Databáze: | OpenAIRE |
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