Effect of resveratrol on experimental non-alcoholic steatohepatitis
| Autor: | Lars Porskjær Christensen, Sara Heebøll, Jacob George, Martin Kreutzfeldt, Niels Jessen, Elias Immanuel Ordell Sundelin, Steen B. Pedersen, Karen Louise Thomsen, Lionel Hebbard, Andrew D. Clouston, Yulia Radko, Mehdi Ramezani-Moghadam, Henning Grønbæk |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Resveratrol Antioxidants Transaminase chemistry.chemical_compound Liver Function Tests Fibrosis Non-alcoholic Fatty Liver Disease Internal medicine Stilbenes medicine Animals Rats Wistar Triglycerides Pharmacology Triglyceride medicine.diagnostic_test Fatty liver food and beverages Organ Size medicine.disease Disease Models Animal Endocrinology Treatment Outcome chemistry Liver Female Steatosis Steatohepatitis Liver function tests |
| Zdroj: | Heebøll, S, Thomsen, K L, Clouston, A, Sundelin, E, Radko, Y, Christensen, L P, Ramezani-Moghadam, M, Kreutzfeldt, M, Pedersen, S B, Jessen, N, Hebbard, L, George, J & Grønbæk, H 2015, ' Effect of resveratrol on experimental non-alcoholic steatohepatitis ', Pharmacological Research, vol. 95-96, pp. 34-41 . https://doi.org/10.1016/j.phrs.2015.03.005 Heebøll, S, Thomsen, K L, Clouston, A, Sundelin, E, Radko, Y, Christensen, L P, Ramezani-Moghadam, M, Martin Kreutzfeldt, M, Pedersen, S B, Jessen, N, Hebbard, L, George, J & Grønbæk, H 2015, ' Effect of resveratrol on experimental non-alcoholic steatohepatitis ', Pharmacological Research, vol. 95-96, pp. 34-41 . https://doi.org/10.1016/j.phrs.2015.03.005 |
| DOI: | 10.1016/j.phrs.2015.03.005 |
| Popis: | Non-alcoholic fatty liver disease and non-alcoholic steatohepatitis (NASH) are increasing clinical problems for which effective treatments are required. The polyphenol resveratrol prevents the development of fatty liver disease in a number of experimental studies. We hypothesized that it could revert steatohepatitis, including hepatic inflammation and fibrosis, in an experimental NASH model. To induce hepatic steatohepatitis, a 65% fat, 2% cholesterol and 0.5% cholate (HFC) diet was fed to rats for 1 or 16 weeks, prior to treatment. Subsequently, the diet was supplemented with resveratrol (approx. 100mg/rat/day) to three intervention groups; week 2-4, 2-7 or 17-22. Treated animals were sacrificed at the end of each intervention period with appropriate control and HFC diet controls. Blood and liver were harvested for analysis. When commenced early, resveratrol treatment partially mitigated transaminase elevations, hepatic enlargement and TNFα induced protein-3 protein expression, but generally resveratrol treatment had no effect on elevated hepatic triglyceride levels, histological steatohepatitis or fibrosis. We observed a slight reduction in Collagen1α1 mRNA expression and no reduction in the mRNA expression of other markers of fibrosis, inflammation or steatosis (TGFβ, TNFα, α2-MG, or SREBP-1c). Resveratrol metabolites were detected in serum, including trans-resveratrol-3-O-sulphate/trans-resveratrol-4'-O-sulphate (mean concentration 7.9μg/ml). Contrary to the findings in experimental steatosis, resveratrol treatment had no consistent therapeutic effect in alleviating manifest experimental steatohepatitis. |
| Databáze: | OpenAIRE |
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