Glucose and galactose transport in Bifidobacterium bifidum DSM 20082
| Autor: | Françoise Gavini, Stéphane Bouquelet, Colette Brassart, Frédéric Krzewinski |
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| Přispěvatelé: | ProdInra, Migration, Unité de recherche d'Écologie et Physiologie du Système Digestif (UEPSD), Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Bifidobacterium bifidum
Chromatography ved/biology [SDV]Life Sciences [q-bio] ved/biology.organism_classification_rank.species Galactose Biological Transport General Medicine Carbohydrate Membrane transport Applied Microbiology and Biotechnology Microbiology [SDV] Life Sciences [q-bio] chemistry.chemical_compound Glucose chemistry Biochemistry Symporter Bifidobacterium Phosphorylation Sugar Galactose transport Lasalocid |
| Zdroj: | Current Microbiology Current Microbiology, Springer Verlag, 1997, 35 (3), pp.175-179 |
| ISSN: | 0343-8651 1432-0991 |
| Popis: | Sugar uptake was measured with 3H-galactose and 14C-glucose. Galactose transport system was not modified by inhibitors of known translocases and did not present a saturation kinetic with high concentration of galactose. Glucose incorporation was inhibited by lasalocid (cation symport inhibitor) and increased by KCl. The kinetic parameters KM and Vmax were respectively 9.16 mM and 26.56 nmol/min/mg cell protein. On the basis of this study, galactose crossed through the membrane by diffusion, and glucose was incorporated by a cation symport which is regulated by K+ ions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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