Inhibitory effect of Saccharomyces cerevisiae extract obtained through ultrasound-assisted extraction on melanoma cells

Autor: Hyunjhung Jhun, Tae-Gyu Lim, Su Jin Eom, Kyung-Mo Song, Min-Cheol Kang, Nam Hyouck Lee
Rok vydání: 2021
Předmět:
Skin Neoplasms
Lysis
Acoustics and Ultrasonics
Saccharomyces cerevisiae
QC221-246
Antineoplastic Agents
02 engineering and technology
010402 general chemistry
01 natural sciences
Inorganic Chemistry
Mice
UAE
ultrasound-assisted extraction
Cyclin D1
Cell Wall
medicine
Animals
Chemical Engineering (miscellaneous)
Environmental Chemistry
Yeast extract
Radiology
Nuclear Medicine and imaging
Original Research Article
Melanoma
QD1-999
Cell Proliferation
Mice
Inbred BALB C
Ultrasound extraction
biology
Cell growth
Chemistry
fungi
Organic Chemistry
Extraction (chemistry)
Acoustics. Sound
food and beverages
UAEY
Ultrasound-assisted extract of yeast
021001 nanoscience & nanotechnology
biology.organism_classification
medicine.disease
Glucan
Yeast
Tumor Burden
0104 chemical sciences
Ultrasonic Waves
Biochemistry
0210 nano-technology
Zdroj: Ultrasonics Sonochemistry, Vol 76, Iss, Pp 105620-(2021)
Ultrasonics Sonochemistry
ISSN: 1350-4177
DOI: 10.1016/j.ultsonch.2021.105620
Popis: Highlights • Ultrasound extraction can be used to optimize the yeast extraction process. • Yeast ultrasonic extracts suppress the growth of melanoma cells. • This green technology can be scaled-up for industrial yeast extraction.
Although the immune enhancing effect of yeast has been widely reported, studies specifically investigating its effects on skin cancer are lacking. Therefore, this study aimed to develop a yeast extract capable of inhibiting melanoma cells using ultrasound technology, which can lyse the cell walls allowing subsequent rapid yeast extraction. To compare the extraction efficiency across different extraction methods, the total yield, as well as total glucan, α-glucan, and β-glucan yields were measured. Ultrasound-assisted extract of yeast (UAEY) was found to effectively inhibit melanoma cell growth and proliferation as well as the expression of cyclin D1 and c-myc, in vitro. Additionally, the extract reduced melanoma tumor volume and cyclin D1 levels in BALB/c nu/nu mice. The optimal extraction conditions were 0.2 M NaOH, 3 h, 70 °C, 20 kHz, and 800 W, resulting in an increased total extraction and β-glucan yields of 73.6% and 7.1%, respectively, compared with that achieved using a conventional chemical (0.5 M NaOH) extraction method. Taken together, the results of this study suggest that UAEY may represent an effective anti-skin cancer agent.
Databáze: OpenAIRE
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