ROCK Y-27632 Inhibitor, Ascorbic Acid, and Trehalose Increase Survival of Human Wharton Jelly Mesenchymal Stem Cells After Cryopreservation
| Autor: | Mahdokht Hossein Aghdaie, Mohamad Reza Azarpira, Seideh Masoomeh Nekoei, Elaheh Esfandiari, Sulmaz Kamalifar, Negar Azarpira, Sadegh Ghorbani-Dalini, Ladan Sadeghi |
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| Rok vydání: | 2020 |
| Předmět: |
Cell Survival
Pyridines Ascorbic Acid Cell Separation 030230 surgery Umbilical cord Cryopreservation Andrology 03 medical and health sciences chemistry.chemical_compound Cryoprotective Agents 0302 clinical medicine Wharton's jelly Cell Adhesion Humans Medicine MTT assay Wharton Jelly Viability assay Protein Kinase Inhibitors Cells Cultured Cell Proliferation rho-Associated Kinases Transplantation business.industry Mesenchymal stem cell Trehalose Cell Differentiation Mesenchymal Stem Cells Ascorbic acid Amides Phenotype medicine.anatomical_structure chemistry business |
| Zdroj: | Experimental and Clinical Transplantation. 18:505-511 |
| ISSN: | 2146-8427 1304-0855 |
| Popis: | Objectives Wharton jelly mesenchymal stem cells are good candidates for application in different aspects of regenerative medicine, and their long-time banking is important. In this study, the effects of trehalose, ascorbic acid, and Y-27632 on proliferation and survival rate of these cells after cryopreservation were investigated. Materials and methods Mesenchymal stem cells were isolated from human umbilical cord Wharton jelly and frozen using a slow-rate cooling process. Different concentrations of trehalose (35, 75, and 125 mM), ascorbic acid (0.06, 0.125, 0.25, and 0.5 mM), and Y-27632 (10 μM) were used to treat culture medium and/or to supplement freezing medium. Assessment of cell viability after thawing was performed using Trypan blue staining, and MTT assay was performed to measure the cell proliferation rate. Results We observed significantly increased postthaw viability, increased cell proliferation, and decreased doubling time of cells when 75 mM trehalose, 0.25 and 0.5 mM ascorbic acid, and 10 mM Y-27632 were used. In addition, increased viability, proliferation, and attachment were observed after 24 hours of pretreatment with these cryoprotective agents and when they were added to conventional freezing medium. Conclusions The use of different cryoprotective agents in culture and freezing media could be useful for long-term storage of Wharton jelly mesenchymal stem cells. |
| Databáze: | OpenAIRE |
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