Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment
Autor: | Yulong Wei, Luqiang Wang, Jihwan Park, Yanqing Gong, Mingyao Li, Robert J. Tower, Leilei Zhong, Fanxin Long, Katalin Susztak, Nathanial Dyment, Chider Chen, Wei Tong, Patrick Seale, Nicholas Holdreith, Wei Yu, Zhen Miao, Ling Qin, Xiaobin Huang, Yejia Zhang, Lutian Yao, Jaimo Ahn, Rojesh Shrestha |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Mouse Mice 0302 clinical medicine Bone Marrow Adipocytes Biology (General) education.field_of_study General Neuroscience food and beverages Cell Differentiation Osteoblast Genomics General Medicine Cell biology medicine.anatomical_structure Adipogenesis 030220 oncology & carcinogenesis osteoblast Medicine Insight Research Article Cell type Stromal cell QH301-705.5 Science Population Bone Marrow Cells Biology adipocyte General Biochemistry Genetics and Molecular Biology 03 medical and health sciences blood vessel medicine Animals single cell RNA-seq Cell Lineage Progenitor cell Bone education Osteoblasts General Immunology and Microbiology fungi Mesenchymal stem cell bone marrow mesenchymal stem cells Mesenchymal Stem Cells Cell Biology 030104 developmental biology Bone marrow Transcriptome |
Zdroj: | eLife, Vol 9 (2020) eLife |
Popis: | Bone marrow mesenchymal lineage cells are a heterogeneous cell population involved in bone homeostasis and diseases such as osteoporosis. While it is long postulated that they originate from mesenchymal stem cells, the true identity of progenitors and their in vivo bifurcated differentiation routes into osteoblasts and adipocytes remain poorly understood. Here, by employing large scale single cell transcriptome analysis, we computationally defined mesenchymal progenitors at different stages and delineated their bi-lineage differentiation paths in young, adult and aging mice. One identified subpopulation is a unique cell type that expresses adipocyte markers but contains no lipid droplets. As non-proliferative precursors for adipocytes, they exist abundantly as pericytes and stromal cells that form a ubiquitous 3D network inside the marrow cavity. Functionally they play critical roles in maintaining marrow vasculature and suppressing bone formation. Therefore, we name them marrow adipogenic lineage precursors (MALPs) and conclude that they are a newly identified component of marrow adipose tissue. |
Databáze: | OpenAIRE |
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