Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment

Autor: Yulong Wei, Luqiang Wang, Jihwan Park, Yanqing Gong, Mingyao Li, Robert J. Tower, Leilei Zhong, Fanxin Long, Katalin Susztak, Nathanial Dyment, Chider Chen, Wei Tong, Patrick Seale, Nicholas Holdreith, Wei Yu, Zhen Miao, Ling Qin, Xiaobin Huang, Yejia Zhang, Lutian Yao, Jaimo Ahn, Rojesh Shrestha
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Mouse
Mice
0302 clinical medicine
Bone Marrow
Adipocytes
Biology (General)
education.field_of_study
General Neuroscience
food and beverages
Cell Differentiation
Osteoblast
Genomics
General Medicine
Cell biology
medicine.anatomical_structure
Adipogenesis
030220 oncology & carcinogenesis
osteoblast
Medicine
Insight
Research Article
Cell type
Stromal cell
QH301-705.5
Science
Population
Bone Marrow Cells
Biology
adipocyte
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
blood vessel
medicine
Animals
single cell RNA-seq
Cell Lineage
Progenitor cell
Bone
education
Osteoblasts
General Immunology and Microbiology
fungi
Mesenchymal stem cell
bone marrow mesenchymal stem cells
Mesenchymal Stem Cells
Cell Biology
030104 developmental biology
Bone marrow
Transcriptome
Zdroj: eLife, Vol 9 (2020)
eLife
Popis: Bone marrow mesenchymal lineage cells are a heterogeneous cell population involved in bone homeostasis and diseases such as osteoporosis. While it is long postulated that they originate from mesenchymal stem cells, the true identity of progenitors and their in vivo bifurcated differentiation routes into osteoblasts and adipocytes remain poorly understood. Here, by employing large scale single cell transcriptome analysis, we computationally defined mesenchymal progenitors at different stages and delineated their bi-lineage differentiation paths in young, adult and aging mice. One identified subpopulation is a unique cell type that expresses adipocyte markers but contains no lipid droplets. As non-proliferative precursors for adipocytes, they exist abundantly as pericytes and stromal cells that form a ubiquitous 3D network inside the marrow cavity. Functionally they play critical roles in maintaining marrow vasculature and suppressing bone formation. Therefore, we name them marrow adipogenic lineage precursors (MALPs) and conclude that they are a newly identified component of marrow adipose tissue.
Databáze: OpenAIRE
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