Optimizing expression of functional HIV envelopes in rVSV-ΔG vaccine vectors

Autor:	Perry J. Tiberio, CR King, Christopher L. Parks, Michael J. Caulfield, Aaron J. Wilson, M Kemelman, Sanjay Phogat, Christy Jurgens, Maoli Yuan, Kevin J. Wright, Simon Hoffenberg, Ivo C. Lorenz, Cesar Boggiano
Rok vydání:	2012
Předmět:	chemistry.chemical_classification viruses Protein domain Nucleic acid sequence Computational biology Biology Bioinformatics ENCODE Infectious Diseases Protein structure chemistry Virology Poster Presentation biology.protein Vero cell Coding region Antibody Glycoprotein
Zdroj:	Retrovirology
ISSN:	1742-4690
DOI:	10.1186/1742-4690-9-s2-p342
Popis:	Methods Using a combination of nucleotide sequence optimization and protein domain swapping, we have generated a panel of novel gene inserts for VSV vectors that encode chimeric HIV-1 and VSV glycoprotein immunogens (EnvG). A stable VERO cell line engineered to express human CD4 and CCR5 was used to rescue rVSV vectors in which the G gene was replaced with coding sequence for several different EnvG proteins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c90eaa380539c14796d3d80646e58035 https://doi.org/10.1186/1742-4690-9-s2-p342 Zobrazit plný text záznamu Full text from SpringerLink