Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1
| Autor: | S. Roy Himes, David A. Hume, Stephen L. Cronau, Timothy Ravasi, R. Tedjo Sasmono, John A. Hamilton, Andrew D. Cook, Achim Ehrnsperger, Rangi Kaushalya Kandane, Michael J. Hickey |
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| Rok vydání: | 2007 |
| Předmět: |
Macrophage colony-stimulating factor
Neutrophils Cellular differentiation Immunology Receptor Macrophage Colony-Stimulating Factor Biology Granulocyte S100A9 Mice Gene expression medicine Animals Immunology and Allergy RNA Messenger Receptor Cells Cultured Reporter gene Gene Expression Profiling Macrophage Colony-Stimulating Factor Macrophages Cell Differentiation Cell Biology Molecular biology Gene expression profiling medicine.anatomical_structure Granulocytes |
| Zdroj: | Journal of Leukocyte Biology. 82:111-123 |
| ISSN: | 1938-3673 0741-5400 |
| DOI: | 10.1189/jlb.1206713 |
| Popis: | The differentiation of macrophages from their progenitors is controlled by macrophage colony-stimulating factor (CSF-1), which binds to a receptor (CSF-1R) encoded by the c-fms proto-oncogene. We have previously used the promoter region of the CSF-1R gene to direct expression of an enhanced green fluorescent protein (EGFP) reporter gene to resident macrophage populations in transgenic mice. In this paper, we show that the EGFP reporter is also expressed in all granulocytes detected with the Gr-1 antibody, which binds to Ly-6C and Ly-6G or with a Ly-6G-specific antibody. Transgene expression reflects the presence of CSF-1R mRNA but not CSF-1R protein. The same pattern is observed with the macrophage-specific F4/80 marker. Based on these findings, we performed a comparative array profiling of highly purified granulocytes and macrophages. The patterns of mRNA expression differed predominantly through granulocyte-specific expression of a small subset of transcription factors (Egr1, HoxB7, STAT3), known abundant granulocyte proteins (e.g., S100A8, S100A9, neutrophil elastase), and specific receptors (fMLP, G-CSF). These findings suggested that appropriate stimuli might mediate rapid interconversion of the major myeloid cell types, for example, in inflammation. In keeping with this hypothesis, we showed that purified Ly-6G-positive granulocytes express CSF-1R after overnight culture and can subsequently differentiate to form F4/80-positive macrophages in response to CSF-1. |
| Databáze: | OpenAIRE |
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