Cyclooxygenase-2 mRNA expression in equine nonglandular and glandular gastric mucosal biopsy specimens obtained before and after induction of gastric ulceration via intermittent feed deprivation
| Autor: | Christopher R. Bellenger, Alan W. Baird, M.T. Ryan, Niamh K. Morrissey |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Pathology medicine.medical_specialty Biopsy Gene Expression Regulation Enzymologic Gastroscopy Gastric mucosa medicine Animals Horses RNA Messenger Stomach Ulcer DNA Primers General Veterinary biology medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Stomach General Medicine Animal Feed Immunohistochemistry digestive system diseases Epithelium Staining medicine.anatomical_structure Real-time polymerase chain reaction Cyclooxygenase 2 Gastric Mucosa biology.protein Female Horse Diseases Antibody Food Deprivation Orchiectomy |
| Zdroj: | American Journal of Veterinary Research. 71:1312-1320 |
| ISSN: | 0002-9645 |
| DOI: | 10.2460/ajvr.71.11.1312 |
| Popis: | Objective—To measure the expression of cyclooxygenase-2 (COX-2) mRNA in gastric biopsy specimens serially obtained from horses before, during, and after an 8-day intermittent feed-deprivation trial and to investigate the mucosal location of COX-2. Animals—9 mixed-breed horses for retrieval of gastric biopsy specimens and 16 additional horses for immunohistochemical analysis. Procedures—Gastric biopsy specimens were obtained from 6 horses; 3 of these horses and 3 more participated in an intermittent feed-deprivation trial 9 weeks later. A quantitative PCR assay was used to determine the amount of COX-2 mRNA in biopsy specimens from nonulcerated and ulcerated gastric mucosa. Immunohistochemical staining of specimens by use of a polyclonal anti–COX-2 antibody was performed on full-thickness postmortem gastric biopsy specimens. Results—COX-2 mRNA was expressed in all glandular gastric mucosal specimens but was only detectable in nonglandular mucosal specimens when ulceration was present or during ulcer healing. Positive staining for COX-2 was present in 12 of 14 nonulcerated glandular mucosal sections. Although such staining was weak or absent in nonulcerated nonglandular sections, stronger staining was evident in regenerating epithelium at the rims of erosions and ulcers. Conclusions and Clinical Relevance—COX-2 was constitutively present in equine glandular gastric mucosa, although its contribution to mucosal protection remains unclear. Our finding of COX-2 mRNA expression in ulcer margins during healing may support a role for the products of this enzyme in mucosal repair. The potential roles of COX-2 should be considered when COX-2–selective inhibitors are prescribed for horses with gastric ulcers. (Am J Vet Res 2010;71:1312–1320) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|