Replacing the Calvin cycle with the reductive glycine pathway in Cupriavidus necator
| Autor: | Max Finger-Bou, Giovanni Scarinci, Nico J. Claassens, Alberto De Maria, Jari Verbunt, Stijn T. de Vries, William Newell, Martí Munar-Palmer, Suzan Yilmaz, Natalia Giner-Laguarda, Charles A. R. Cotton, Guillermo Bordanaba-Florit, Arren Bar-Even, Lukas Friedeheim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Glycine biosynthesis Cupriavidus necator Glycine Bioengineering Tetrahydrofolate 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Microbial electrosynthesis 010608 biotechnology Formate Biomass Photosynthesis Glycine cleavage system 030304 developmental biology 0303 health sciences Glycine metabolism biology food and beverages Assimilation (biology) Metabolism biology.organism_classification Bioproduction One-carbon metabolism chemistry Biochemistry Pathway activity Biotechnology |
| Zdroj: | Metabolic Engineering Metabolic Engineering, 62, 30-41 Metabolic Engineering 62 (2020) |
| ISSN: | 1096-7176 |
| Popis: | Formate can be directly produced from CO2 and renewable electricity, making it a promising microbial feedstock for sustainable bioproduction. Cupriavidus necator is one of the few biotechnologically-relevant hosts that can grow on formate, but it uses the Calvin cycle, the high ATP cost of which limits biomass and product yields. Here, we redesign C. necator metabolism for formate assimilation via the synthetic, highly ATP-efficient reductive glycine pathway. First, we demonstrate that the upper pathway segment supports glycine biosynthesis from formate. Next, we explore the endogenous route for glycine assimilation and discover a wasteful oxidation-dependent pathway. By integrating glycine biosynthesis and assimilation we are able to replace C. necator's Calvin cycle with the synthetic pathway and achieve formatotrophic growth. We then engineer more efficient glycine metabolism and use short-term evolution to optimize pathway activity. The final growth yield we achieve (2.6 gCDW/mole-formate) nearly matches that of the WT strain using the Calvin Cycle (2.9 gCDW/mole-formate). We expect that further rational and evolutionary optimization will result in a superior formatotrophic C. necator strain, paving the way towards realizing the formate bio-economy. |
| Databáze: | OpenAIRE |
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