Combined analysis of galectin-3 and BRAFV600E improves the accuracy of fine-needle aspiration biopsy with cytological findings suspicious for papillary thyroid carcinoma
| Autor: | Pasquale Vallefuoco, D. Posca, Paolo Piero Limone, Maria Rosaria Sapio, Maurilio Deandrea, Giancarlo Troncone, Alessia Caleo, Gianfranco Fenzi, Guido Rossi, Mario Vitale, Manuela Motta, Anna Guerra |
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| Přispěvatelé: | Sapio, Mr, Guerra, Anna, Posca, D, Limone, Pp, Deandrea, M, Motta, M, Troncone, Giancarlo, Caleo, Alessia, Vallefuoco, Pasquale, Rossi, Guido, Fenzi, Gianfranco, Vitale, Mario, Guerra, A, Caleo, C, Vallefuoco, P |
| Rok vydání: | 2007 |
| Předmět: |
Thyroid nodules
Proto-Oncogene Proteins B-raf Cancer Research medicine.medical_specialty Pathology endocrine system diseases CARCINOMA Endocrinology Diabetes and Metabolism Galectin 3 Biopsy Fine-Needle Malignancy Polymorphism Single Nucleotide Sensitivity and Specificity Thyroid carcinoma Endocrinology Predictive Value of Tests Biopsy medicine Humans Thyroid Neoplasms medicine.diagnostic_test business.industry Adenine Thyroid medicine.disease Adenocarcinoma Papillary medicine.anatomical_structure Fine-needle aspiration Oncology Amino Acid Substitution CAMK Predictive value of tests Adenocarcinoma TIROIDE Radiology business Thymine |
| Zdroj: | Endocrine-related cancer. 14(4) |
| ISSN: | 1351-0088 |
| Popis: | Ten to fifteen percent of fine-needle aspiration biopsy (FNAB) of thyroid nodules are indeterminate. Galectin-3 (Gal-3) and the oncogene BRAFV600E are markers of malignancy useful to improve FNAB accuracy. The objective of this study was to determine whether the combined analysis of Gal-3 and BRAFV600E expression in thyroid aspirates could improve the diagnosis in FNAB with suspicious cytological findings. Two hundred and sixty-one surgical thyroid tissues and one hundred and forty-four thyroid aspirates were analyzed for the presence of the two markers. In surgical specimens, Gal-3 expression was present in 27.4% benign nodules, 91.9% papillary (PTC) and 75% follicular (FTC) thyroid carcinomas. BRAFV600E was not detected in 127 benign nodules, as well as in 32 FTCs, while was found in 42.9% PTC. No correlation was found between BRAF mutation and Gal-3 expression. Forty-seven consecutive FNAB suspicious for PTC were analyzed for the presence of the two markers. Of these nodules, 23 were benign at histology, 6 were positive for Gal-3, none displayed BRAFV600E, and 17 were negative for both the markers. Twenty suspicious nodules were diagnosed as PTC and four FTCs at histology. Of these 24 carcinomas, 9 resulted positive for BRAFV600E, 17 for Gal-3, and 22 for one or both the markers. The sensitivity, specificity, and accuracy for the presence of Gal-3 and/or BRAFV600E were significantly higher than those obtained for the two markers alone. Notably, the negative predictive value increased from 70.8 to 89.5%. In conclusion, the combined detection of Gal-3 and BRAFV600E improves the diagnosis in FNAB with cytological findings suspicious for PTC and finds clinical application in selected cases. |
| Databáze: | OpenAIRE |
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