Puerarin exerts antipyretic effect on lipopolysaccharide-induced fever in rats involving inhibition of pyrogen production from macrophages

Autor: Carlos Feleder, Ji-Ai Yin, Mei Liu, Yubin Luo, Zhifeng Wei, Xiujuan Yao, Yue Dai, Yufeng Xia
Rok vydání: 2012
Předmět:
Lipopolysaccharides
Male
Time Factors
Transcription
Genetic
Lipopolysaccharide
medicine.medical_treatment
Pharmacology
law.invention
Rats
Sprague-Dawley
Mice
chemistry.chemical_compound
Puerarin
law
Drug Discovery
Medicine
Phosphorylation
Prostaglandin E2
Reverse Transcriptase Polymerase Chain Reaction
NF-kappa B
Tumor necrosis factor alpha
Inflammation Mediators
Mitogen-Activated Protein Kinases
Injections
Intraperitoneal
Body Temperature Regulation
Signal Transduction
medicine.drug
Antipyretics
Fever
Cell Survival
Blotting
Western
Intraperitoneal injection
Cell Line
Nitric oxide
Animals
RNA
Messenger
Antipyretic
Plants
Medicinal
Dose-Response Relationship
Drug
business.industry
Macrophages
Isoflavones
Rats
Disease Models
Animal
Gene Expression Regulation
chemistry
Immunology
business
Phytotherapy
Zdroj: Journal of Ethnopharmacology. 141:322-330
ISSN: 0378-8741
DOI: 10.1016/j.jep.2012.02.038
Popis: Ethnopharmacological relevance Puerarin is the most abundant isoflavonoid in Radix Puerariae (Gegen), which has been prescribed as a medicinal herb for treating fever in China for a long history. Aim of the study The present study aimed at evaluating the antipyretic effect of puerarin and revealing the related mechanisms. Materials and methods Lipopolysaccharide (LPS)-induced fever in rats was used to assess the antipyretic effect of puerarin. After an intraperitoneal injection of LPS (100 μg/kg), body temperature was tested every 30 min up to 8 h. Different doses of puerarin (25, 50, 100 mg/kg) were intraperitoneally administered 30 min before LPS injection. In vitro, LPS-stimulated RAW 264.7 cells were treated with various concentrations of puerarin (25–200 μM). The pyrogenic mediators, including interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), prostaglandin E2 (PGE2) and nitric oxide (NO), were examined on both transcription and expression levels. Furthermore, the influences of the activation of nuclear factor-kappa B (NF-κB) and the phosphorylation of mitogen-activated protein kinases (MAPKs) by puerarin were assayed by western blot. Results The intraperitoneal administration of puerarin at test doses clearly demonstrated apparent antipyretic effect through the declines in body temperature elevated by LPS in rats. The in vitro data showed that puerarin inhibited the production of IL-1β, TNF-α, IL-6, PGE2 and NO; moreover, the RT-PCR analysis and the western blot analysis indicated that puerarin regulated the transcriptional level via suppression of NF-κB activation and blockade of MAPK signal pathway. Conclusions In summary, the antipyretic property of puerarin might result, at least in part, from an inhibition of endogenous pyrogen production and expression. Taken in this sense, our findings provide an explanation for puerarin acting as an important constituent in Gegen, thus, provide scientific basis for the wide use of Radix Puerariae in China as a traditional antipyretic.
Databáze: OpenAIRE
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