| Autor: |
Sakthivel Kumaravel, Guo-Rong Luo, Sheng-Tung Huang, Hsin-Yi Lin, Chun-Mao Lin, Yu-Chieh Lee |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Biosensors and Bioelectronics. 203:114049 |
| ISSN: |
0956-5663 |
| DOI: |
10.1016/j.bios.2022.114049 |
| Popis: |
Aminopeptidase N (APN/CD13) plays an important role in the growth and metastasis, of tumor, and is a potential biomarker for the post-treatment surveillance of cancer reoccurrence and progression of various malignancies. Thus, we have designed and prepared a convenient and ultrasensitive APN-targeting activity-based ratiometric electrochemical molecular substrate (Ala-AFC) for direct real-time monitoring of APN activity in biosamples. The APN in our experiment was used to hydrolyze the alanine moiety of the Ala-AFC probe and, as a result of this hydrolysis, realize concomitantly a cascade reaction to unmask the electrochemical reporter N-alkylated amino ferrocene (AAF). The Ala-AFC probe exhibited high sensitivity with a wide detection range of 0.05-110 ng mL |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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