Development and Characterization of an HPV Type-16 Specific Modified DNA Aptamer for the Improvement of Potency Assays
| Autor: | Mary Shank-Retzlaff, Thorsten Verch, Jeremiah J. Trausch |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Aptamer Enzyme-Linked Immunosorbent Assay 01 natural sciences Epitope Analytical Chemistry Antigen-Antibody Reactions Epitopes 03 medical and health sciences Antigen medicine Humans Papillomavirus Vaccines Neutralizing antibody Antigens Viral Human papillomavirus 16 biology Chemistry Gardasil 010401 analytical chemistry Aptamers Nucleotide Molecular biology 0104 chemical sciences 030104 developmental biology Vaccine Potency Capsid biology.protein Antibody medicine.drug |
| Zdroj: | Analytical Chemistry. 89:3554-3561 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Measuring vaccine potency is critical for vaccine release and is often accomplished using antibody-based ELISAs. Antibodies can be associated with significant drawbacks that are often overlooked including lot-to-lot variability, problems with cell-line maintenance, limited stability, high cost, and long discovery lead times. Here, we address many of these issues through the development of an aptamer, known as a slow off-rate modified DNA aptamer (SOMAmer), which targets a vaccine antigen in the human papillomavirus (HPV) vaccine Gardasil. The aptamer, termed HPV-07, was selected to bind the Type 16 virus-like-particle (VLP) formed by the self-assembling capsid protein L1. It is capable of binding with high sensitivity (EC50 of 0.1 to 0.4 μg/mL depending on assay format) while strongly discriminating against other VLP types. The aptamer competes for binding with the neutralizing antibody H16.V5, indicating at least partial recognition of a neutralizing and clinically relevant epitope. This makes it a usefu... |
| Databáze: | OpenAIRE |
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