Follistatin-like 1 Suppresses Sensory Afferent Transmission by Activating Na+,K+-ATPase
| Autor: | Jun-Ru Yao, Rohini Kuner, Lan Bao, Qiong Wang, Hua-Sheng Xiao, Mingyan Yu, Yu-Qiu Zhang, Kai-Cheng Li, Xiang Gao, Kai-Hua Zhang, Mei Han, Yan-Qing Zhong, Xu Zhang, Ying-Jin Lu, Fang-Xiong Zhang, Xiao-Li Ma, Jin-Yuan Wang, Feng Wang, Li-Bo Lin, Chang-Lin Li |
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| Rok vydání: | 2011 |
| Předmět: |
Follistatin-Related Proteins
Patch-Clamp Techniques Sensory Receptor Cells Neuroscience(all) Blotting Western Presynaptic Terminals Neurotransmission Biology Inhibitory postsynaptic potential Synaptic vesicle Synaptic Transmission Mice Dorsal root ganglion Ganglia Spinal Chlorocebus aethiops medicine Animals Patch clamp Axon Na+/K+-ATPase Cells Cultured Mice Knockout Analysis of Variance General Neuroscience Hyperpolarization (biology) respiratory system Blotting Northern Immunohistochemistry Cell biology Rats medicine.anatomical_structure COS Cells Sodium-Potassium-Exchanging ATPase Neuroscience |
| Zdroj: | Neuron. 69(5):974-987 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/j.neuron.2011.01.022 |
| Popis: | SummaryExcitatory synaptic transmission is modulated by inhibitory neurotransmitters and neuromodulators. We found that the synaptic transmission of somatic sensory afferents can be rapidly regulated by a presynaptically secreted protein, follistatin-like 1 (FSTL1), which serves as a direct activator of Na+,K+-ATPase (NKA). The FSTL1 protein is highly expressed in small-diameter neurons of the dorsal root ganglion (DRG). It is transported to axon terminals via small translucent vesicles and secreted in both spontaneous and depolarization-induced manners. Biochemical assays showed that FSTL1 binds to the α1 subunit of NKA and elevates NKA activity. Extracellular FSTL1 induced membrane hyperpolarization in cultured cells and inhibited afferent synaptic transmission in spinal cord slices by activating NKA. Genetic deletion of FSTL1 in small DRG neurons of mice resulted in enhanced afferent synaptic transmission and sensory hypersensitivity, which could be reduced by intrathecally applied FSTL1 protein. Thus, FSTL1-dependent activation of NKA regulates the threshold of somatic sensation. |
| Databáze: | OpenAIRE |
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