Reduced rate of ATP catabolism and the enhanced amino acid incorporating activity of regenerating rat liver microsomes
| Autor: | J. Mager, A. Halbreich, Judith Hamburger-Heyd |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
Phenylalanine
Biophysics Biology In Vitro Techniques Biochemistry Ribosome Rat liver microsomes Adenosine Triphosphate Microsomes Protein biosynthesis Animals Amino Acids Molecular Biology chemistry.chemical_classification Carbon Isotopes Catabolism Cell Biology Amino acid Liver Regeneration Rats Membrane chemistry Liver Cytoplasm Protein Biosynthesis Microsome |
| Zdroj: | Biochemical and biophysical research communications. 26(4) |
| ISSN: | 0006-291X |
| Popis: | It has been reported by several investigators ( Hultin and Von der Decken, 1958 ; Hoagland et al. , 1964 ; Cammarano et al. , 1965 ) that the ability of cell-free liver preparations to carry out amino acid incorporation into protein increases markedly in the course of the vigorous regeneration following subtotal hepatectomy. The elevated activity was shown to be attributable both to the microsomal particles and to the soluble cytoplasmic fraction ( Hoagland and Askonas, 1963 ; (Cammarano et al. , 1965) . On the other hand, ribosomes derived from normal and regenerating liver proved to be equally efficient in mediating amino acid incorporation (Cammarano et al. , 1965) . It was inferred that the microsomal membranes of the normal liver contain, in excess over the amount present in the regenerating microsomes, a factor that inhibits protein synthesis Hoagland et al. , 1964 ; Cammarano et al. , 1965 ). The results of the present study, though consistent with the above observations, suggest, however, that the enhanced amino acid incorporation capacity of the regenerating-liver microsomes is largely determined by their diminished ATP catabolism. |
| Databáze: | OpenAIRE |
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