Time-resolved NMR monitoring of tRNA maturation
| Autor: | Stefanie Kellner, Matthias Heiss, Carine Tisné, Alexandre Gato, Marjorie Catala, Pierre Barraud |
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| Přispěvatelé: | Expression Génétique Microbienne (EGM (UMR_8261 / FRE_3630)), Institut de biologie physico-chimique (IBPC), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Ludwig-Maximilians-Universität München (LMU), Institut de biologie physico-chimique (IBPC (FR_550)) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Models Molecular TRNA modification Magnetic Resonance Spectroscopy Saccharomyces cerevisiae Proteins Time Factors Science General Physics and Astronomy 02 engineering and technology Saccharomyces cerevisiae 010402 general chemistry 01 natural sciences General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences RNA Transfer Phe RNA Transfer Gene expression RNA Processing Post-Transcriptional lcsh:Science ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences tRNA Methyltransferases Multidisciplinary Base Sequence Chemistry RNA General Chemistry Nuclear magnetic resonance spectroscopy 021001 nanoscience & nanotechnology RNA modification Yeast 0104 chemical sciences [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Nucleic acids [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] 030104 developmental biology Order (biology) Biochemistry Transfer RNA Nucleic Acid Conformation lcsh:Q 0210 nano-technology RNA biosynthesis TRNA maturation Solution-state NMR |
| Zdroj: | Nature Communications Nature Communications, Nature Publishing Group, 2019, 10 (1), ⟨10.1038/s41467-019-11356-w⟩ Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-11356-w⟩ |
| Popis: | Although the biological importance of post-transcriptional RNA modifications in gene expression is widely appreciated, methods to directly detect their introduction during RNA biosynthesis are rare and do not easily provide information on the temporal nature of events. Here, we introduce the application of NMR spectroscopy to observe the maturation of tRNAs in cell extracts. By following the maturation of yeast tRNAPhe with time-resolved NMR measurements, we show that modifications are introduced in a defined sequential order, and that the chronology is controlled by cross-talk between modification events. In particular, we show that a strong hierarchy controls the introduction of the T54, Ψ55 and m1A58 modifications in the T-arm, and we demonstrate that the modification circuits identified in yeast extract with NMR also impact the tRNA modification process in living cells. The NMR-based methodology presented here could be adapted to investigate different aspects of tRNA maturation and RNA modifications in general. Transfer RNA (tRNA) is regulated by RNA modifications. Here the authors employ time-resolved NMR to monitor modifications of yeast tRNAPhe in cellular extracts, revealing a sequential order and cross-talk between modifications. |
| Databáze: | OpenAIRE |
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