Inactivation of thenodHgene inSinorhizobiumsp. BR816 enhances symbiosis withPhaseolus vulgarisL
| Autor: | Anja Croonenborghs, Christel Verreth, Ellen Luyten, Maxime Ndayizeye, Jos Vanderleyden, Carla Snoeck, Jan Michiels, Esperanza Martínez-Romero, Roseline Remans |
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| Rok vydání: | 2007 |
| Předmět: |
Rhizobiaceae
Molecular Sequence Data Sinorhizobium Microbiology Rhizobia Nod factor Sulfation Bacterial Proteins Microscopy Electron Transmission Symbiosis Nitrogen Fixation Genetics Molecular Biology Phaseolus biology food and beverages Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition biology.organism_classification Biochemistry Multigene Family Mutation bacteria Rhizobium Sulfotransferases Oxidoreductases Root Nodules Plant |
| Zdroj: | FEMS Microbiology Letters. 266:210-217 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2006.00521.x |
| Popis: | Sulfate modification on Rhizobium Nod factor signaling molecules is not a prerequisite for successful symbiosis with the common bean (Phaseolus vulgaris L.). However, many bean-nodulating rhizobia, including the broad host strain Sinorhizobium sp. BR816, produce sulfated Nod factors. Here, we show that the nodH gene, encoding a sulfotransferase, is responsible for the transfer of sulfate to the Nod factor backbone in Sinorhizobium sp. BR816, as was shown for other rhizobia. Interestingly, inactivation of nodH enables inoculated bean plants to fix significantly more nitrogen under different experimental setups. Our studies show that nodH in the wild-type strain is still expressed during the later stages of symbiosis. This is the first report on enhanced nitrogen fixation by blocking Nod factor sulfation. |
| Databáze: | OpenAIRE |
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