Identifying probiotic characteristics of Lactobacillus crispatus isolated from the vagina
| Autor: | Ümit Can Erim, Merih Kivanc, Fatma Koc, Sevda Er |
|---|---|
| Přispěvatelé: | Anadolu Üniversitesi |
| Jazyk: | angličtina |
| Předmět: |
medicine.drug_class
Antibiotics lcsh:RS1-441 030226 pharmacology & pharmacy 01 natural sciences law.invention Microbiology Lactobacillus crispatus/probiotic lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound Probiotic 0302 clinical medicine law medicine Lactic acid bacteria Vaginal flora Lactobacillus crispatus biology Sulfamethoxazole biology.organism_classification Trimethoprim 0104 chemical sciences Lactic acid 010404 medicinal & biomolecular chemistry chemistry Bacteria medicine.drug |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences, Vol 55 Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e17507 Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e17507 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2175-9790 0004-8801 1984-8250 |
| Popis: | KOC, Fatma/0000-0002-0368-4028 WOS: 000488016800001 Lactobacilli prevent overproduction of pathogenic microorganisms and contribute protecting vaginal microbiota. Many probiotic microorganisms are categorized as Lactic Acid Bacteria. in this study, it was aimed identifying probiotic characteristics of Lactobacillus crispatus isolated from the vagina of a healthy woman. For this purpose, lactic acid, hydrogen peroxide and proteolytic activity quantities and auto-aggregation, co-aggregation and hydrophobicity abilities of Lactobacillus crispatus, which has been isolated and identified by 16s rRNA sequence analysis, were determined. Additionally, bile salt and acid resistance, along with antibiotic susceptibility of Lactobacillus crispatus were analyzed by the end of 3 hours. Lactic acid, hydrogen peroxide and proteolytic activity quantities of Lactobacillus crispatus were measured 2.275%, 0.334 +/- 0.075 mu g/mL and 2.131 +/- 0,000 mg/mL respectively. the findings include existence of co-aggregation and auto-aggregation ability, but not hydrophobicity. By the end of 3 hours, the viability was preserved in 0.1% and 0.3% bile salt medium and, at pH 3. L. crispatus exhibited resistance to methicillin, metronidazole, oxacillin, and sulfamethoxazole + trimethoprim, but the bacteria exhibited susceptibility to tested the other antibiotics. This study will make an important contribution to the literature about probiotic characteristics of L. crispatus and our strain isolated from the vagina might be considered as a candidate probiotic. Anadolu of University [1305F089 / 2016] This work was supported by a grant from the Anadolu of University and Research, within the research project 1305F089 / 2016. |
| Databáze: | OpenAIRE |
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