A comparison of competitive enzyme immunoassay and precipitin inhibition tests in the analysis of polysaccharide antigenic determinants of oral streptococci
| Autor: | Yasuhiko Satomi, Katsuhiko Hirota, Fusao Ota, Terushige Kawata, Hideaki Nagamune, Komei Fukui, Masahiko Yasuoka, Sonia Senesi, Yasuyuki Akiyama |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
medicine.drug_class
Immunology Monoclonal antibody Epitope Microbiology Immunoenzyme Techniques Epitopes Mice Antigen medicine Animals Immunology and Allergy chemistry.chemical_classification Antigens Bacterial Mice Inbred BALB C Mouth biology medicine.diagnostic_test Polysaccharides Bacterial Antibodies Monoclonal Streptococcus Hydrogen-Ion Concentration biology.organism_classification Precipitin Precipitin Tests Streptococcus mutans Enzyme chemistry Evaluation Studies as Topic Immunoassay biology.protein Antibody |
| Zdroj: | Journal of Immunological Methods. 124:259-266 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(89)90362-1 |
| Popis: | A rapid and convenient method of competitive enzyme immunoassay (EIA) was explored for the immunological analysis by MAbs of polysaccharide antigens prepared from the mutans group of streptococci, and optimal conditions for it were established. These included the concentrations of antigen, monoclonal antibodies (MAbs) and labelled antibody, temperature, pH and reaction time. The results were compared with those obtained by precipitin inhibition tests using the classical quantitative precipitin reaction, and they were found to be consistent with the latter method. It was concluded that the EIA system can be extended to the immunological study of polysaccharide antigen s of oral streptococci using MAbs which perform poorly in precipitation reactions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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