Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detection

Autor:	H. Murphy, Pagona Papakonstantinou, Martin McMullan, Arzum Erdem, Hakan Karadeniz, Surbhi Sharma
Rok vydání:	2010
Předmět:	Streptavidin Nanotube Materials science DNA–DNA hybridization Analytical chemistry Combinatorial chemistry Analytical Chemistry Dielectric spectroscopy chemistry.chemical_compound chemistry Electrode Electrochemistry Graphite Biosensor DNA
Zdroj:	Electroanalysis. 22:611-617
ISSN:	1521-4109 1040-0397
DOI:	10.1002/elan.200900436
Popis:	Disposable graphite pencil electrodes (PGE) modified with multiwalled carbon nanotubes (MWCNTs)-streptavidin (STR) conjugates were used for electrochemical monitoring of label-free DNA hybridization. The surface morphology of PGE electrode before and after hybridization was characterized by scanning electron microscopy. Electrochemical impedance spectroscopy was used to monitor each step of the construction of the DNA biosensor. The biosensor was demonstrated to have excellent selectivity, being able to differentiate complementary sequences from a noncomplementary ones and in addition select the target sequence of DNA from a mixture of other DNA without loss in current sensitivity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8f77d0a79f5906a2f9f966c9b8b46e3 https://doi.org/10.1002/elan.200900436 Zobrazit plný text záznamu Plný text