Preparation and Characterization of an Oral Vaccine Formulation Using Electrosprayed Chitosan Microparticles
| Autor: | Danai-Anastasia Panou, Ioannis S. Chronakis, Line Hagner Nielsen, Ana Carina Loureiro Mendes, Jorge Alberto Sevilla Moreno, Karen Boutrup Stephansen, Anja Boisen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Ovalbumin medicine.medical_treatment Chemistry Pharmaceutical Drug Compounding quil-A Pharmaceutical Science Administration Oral 02 engineering and technology Aquatic Science Quillaja Saponins Cell Line Chitosan 03 medical and health sciences chemistry.chemical_compound Acetic acid Drug Delivery Systems SDG 3 - Good Health and Well-being Dynamic light scattering Drug Discovery Mucoadhesion medicine Zeta potential Animals Humans Particle Size Ecology Evolution Behavior and Systematics particles Vaccines Chromatography Ecology ovalbumin General Medicine Buffer solution 021001 nanoscience & nanotechnology Microspheres Solvent 030104 developmental biology chemistry polysaccharide electrohydrodynamics 0210 nano-technology Agronomy and Crop Science Adjuvant Chickens |
| Zdroj: | Moreno, J A S, Panou, D-A, Stephansen, K, Chronakis, I S, Boisen, A, Mendes, A C L & Nielsen, L H 2018, ' Preparation and Characterization of an Oral Vaccine Formulation Using Electrosprayed Chitosan Microparticles ', AAPS PharmSciTech, vol. 19, no. 8, pp. 3770–3777 . https://doi.org/10.1208/s12249-018-1190-1 |
| ISSN: | 1530-9932 |
| Popis: | Chitosan particles loaded with the antigen ovalbumin (OVA) and the adjuvant Quil-A were produced by electrospray, using mixtures of water/ethanol/acetic acid as a solvent. Three different chitosans designed as HMC+70, HMC+85, and HMC+90 (called as 705010, 855010, and 905010) were tested and its efficacy to be used in oral vaccine delivery applications was investigated. The morphology, size, and zeta potential of the produced particles were investigated, together with the encapsulation efficiency and release of OVA from the three chitosan formulations. Moreover, the mucoadhesion and cytotoxicity of the chitosan microparticles was examined. All the three formulations with OVA and Quil-A were in the micrometer size range and had a positive zeta potential between 46 and 75 mV. Furthermore, all the three formulations displayed encapsulation efficiencies above 80% and the release of OVA over a period of 80 h was observed to be between 38 and 47%. None of the developed formulations exhibited high mucoadhesive properties, either cytotoxicity. The formulation prepared with HMC+70, OVA, and Quil-A had the highest stability within 2 h in buffer solution, as measured by dynamic light scattering. The electrosprayed formulation consisting of HMC+70 with OVA and Quil-A showed to be the most promising as an oral vaccine system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink