Genetic Characterization of Clostridium botulinum Associated with Type B Infant Botulism in Japan
| Autor: | Masafumi Mukamoto, Kaoru Umeda, Shunji Kozaki, Tomoko Kohda, Yoshiyuki Seto |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Botulinum Toxins Genotype Clostridium botulinum type B Molecular Sequence Data Mutation Missense Biology medicine.disease_cause Polymerase Chain Reaction law.invention Microbiology Japan law Clostridium botulinum Pulsed-field gel electrophoresis medicine Cluster Analysis Humans Botulism Botulinum Toxins Type A Genotyping Phylogeny Polymerase chain reaction Molecular Epidemiology Sequence Homology Amino Acid Molecular epidemiology Infant Botulism Infant Bacteriology Sequence Analysis DNA medicine.disease DNA Fingerprinting Virology Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Blotting Southern Food Microbiology Female |
| Zdroj: | Journal of Clinical Microbiology. 47:2720-2728 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | The 15 proteolytic Clostridium botulinum type B strains, including 3 isolates associated with infant botulism in Japan, were genetically characterized by phylogenetic analysis of b oNT/B gene sequences, genotyping, and determination of the boNT/B gene location by using pulsed-field gel electrophoresis (PFGE) for molecular epidemiological analysis of infant botulism in Japan. Strain Osaka05, isolated from a case in 2005, showed a unique boNT/B gene sequence and was considered to be a new BoNT/B subtype by phylogenetic analysis. Strain Osaka06, isolated from a case in 2006, was classified as the B2 subtype, the same as strain 111, isolated from a case in 1995. The five isolates associated with infant botulism in the United States were classified into the B1 subtype. Isolates from food samples in Japan were divided into the B1 and the B2 subtypes, although no relation with infant botulism was shown by PFGE genotyping. The results of PFGE and Southern blot hybridization with undigested DNA suggested that the boNT/B gene is located on large plasmids (approximately 150 kbp, 260 kbp, 275 kbp, or 280 kbp) in five strains belonging to three BoNT/B subtypes from various sources. The botulinum neurotoxin (BoNT) of Osaka05 was suggested to have an antigenicity different from the antigenicities of BoNT/B1 and BoNT/B2 by a sandwich enzyme-linked immunosorbent assay with the recombinant BoNT/B-C-terminal domain. We established a multiplex PCR assay for BoNT/B subtyping which will be useful for epidemiological studies of type B strains and the infectious diseases that they cause. |
| Databáze: | OpenAIRE |
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