Effects of insulin-like growth factor-1 on endoplasmic reticulum stress and autophagy in rat gastric smooth muscle cells cultured at different glucose concentrations in vitro
| Autor: | Xue-Sen Fang, Jun-Yu Guo, Mo-Han Zhang, Zheng Jin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty medicine.medical_treatment Myocytes Smooth Muscle Clinical Biochemistry In Vitro Techniques CHOP Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences Insulin-like growth factor 0302 clinical medicine Western blot In vivo Internal medicine Autophagy medicine Animals Insulin-Like Growth Factor I Molecular Biology Cells Cultured Smooth muscle tissue medicine.diagnostic_test Chemistry Endoplasmic reticulum Growth factor Stomach Cell Biology General Medicine Endoplasmic Reticulum Stress Rats Glucose 030104 developmental biology Endocrinology medicine.anatomical_structure 030220 oncology & carcinogenesis Signal Transduction |
| Zdroj: | Molecular and Cellular Biochemistry. 451:11-20 |
| ISSN: | 1573-4919 0300-8177 |
| DOI: | 10.1007/s11010-018-3388-7 |
| Popis: | The purpose of the study was to observe changes in endoplasmic reticulum stress (ERS)- and autophagy-related proteins in gastric smooth muscle tissues of diabetic rats with gastroparesis, investigate the effect of insulin-like growth factor 1 (IGF-1) on ERS and autophagy in rat gastric smooth muscle cells cultured under different glucose concentrations, and explore the influence of IGF-1 on development of diabetic gastroparesis (DGP). After establishing a rat model of DGP, rats were divided into normal control (NC) and 6-week diabetic model (DM6W) groups. Expression of ERS-related and autophagy-related proteins was detected by western blot analysis and immunofluorescence assay in rat gastric smooth muscle tissue and in vitro-cultured rat gastric smooth muscle cells exposed to different glucose concentrations and treatment with IGF-1 for 24 or 48 h. Changes in glucose-regulated-protein-78 (GRP78), growth arrest and DNA damage-inducible gene 153 (CHOP), and microtubule-associated protein 1A/1B light chain 3B (LC3) expression levels were detected by western blot analysis, and GRP78 and LC3 expression were examined by confocal laser-scanning microscopy. In vivo expression levels of GRP78, CHOP, and LC3 were significantly higher in the DM6W group compared with the NC group (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink