Curvularia lunata , a New Source of Cytochalasin B
| Autor: | Richard J. Cole, John M. Wells, H. C. Cutler, D. H. Spalding |
|---|---|
| Rok vydání: | 1981 |
| Předmět: | |
| Zdroj: | Applied and Environmental Microbiology. 41:967-971 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | A biologically active metabolite was found in crude extracts of Curvularia lunata (Wakker) Boedijn (ATCC 34690) isolated from decayed tissues of litchi fruit ( Litchi chinensis Sonn.). The fungus was grown on a shredded wheat-yeast extract-sucrose medium, and cultures were extracted with chloroform after 3 weeks of growth at 21°C. Chloroform extracts were toxic to day-old cockerels and caused abnormal distortion of wheat coleoptile segments. The major toxin had a 50% lethal dose of 700 mg/kg (oral dose) and was a colorless crystalline material with a melting point of 218°C. Elemental and high-resolution mass spectral analyses indicated a formula of C 29 H 37 NO 5 and a molecular weight of 479.62. The crystalline preparation was identified as 97% cytochalasin B and 3% cytochalasin A. The yield of cytochalasin B from C. lunata cultures grown on 4.5 kg of shredded wheat and 9 liters of yeast extract-sucrose medium was 6.24 g of purified material. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|