Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice
| Autor: | Haibo Feng, Lu Guan, Lihua Yuan, Hong Qiu, Zhenhui Song, Juan Liu, Jing Fan, Xingfa Han, Zhiqiang Yan, Xiaogang Du, Zhenhua Wang |
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| Rok vydání: | 2015 |
| Předmět: |
Antibodies
Viral Lymphocyte Activation Biochemistry Immunoglobulin G Microbiology Epitopes Mice Immune system Adjuvants Immunologic Phagocytosis Polysaccharides T-Lymphocyte Subsets Structural Biology Spectroscopy Fourier Transform Infrared Splenocyte Animals IL-2 receptor Molecular Biology Interleukin 4 CD86 CD40 biology FOXP3 Viral Vaccines Dendritic Cells General Medicine Immunity Humoral Disease Models Animal Foot-and-Mouth Disease Virus Foot-and-Mouth Disease Macrophages Peritoneal biology.protein Cytokines Female Spleen Apiaceae |
| Zdroj: | International Journal of Biological Macromolecules. 78:405-416 |
| ISSN: | 0141-8130 |
| Popis: | Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination. The average molecular weight (Mw) of the CVPS was 968.31 kDa. The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively. Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers. Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells. Additionally, CVPS enhanced CD40(+), CD80(+), and CD86(+) expression on DCs. Moreover, CVPS upregulated MHC-I/II, TLR-2/4 mRNA levels. In contrast, CVPS downregulated TGF-β mRNA expression and the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells. Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency. |
| Databáze: | OpenAIRE |
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