Baboon spermatology: basic assessment and reproducibility in olive baboons (Papio anubis)
| Autor: | Carl Spiessens, Daniel C. Chai, Thomas M. D’Hooghe, Nicholas M. Kiulia, Jason M. Mwenda, Atunga Nyachieo |
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| Rok vydání: | 2012 |
| Předmět: |
Male
endocrine system medicine.medical_treatment Semen analysis Papio anubis Electroejaculation Andrology Reference Values biology.animal medicine Animals Prospective Studies Sperm motility In vitro fertilisation General Veterinary biology medicine.diagnostic_test Reproducibility of Results Hydrogen-Ion Concentration Spermatozoa Sperm Semen Analysis Sperm Motility Animal Science and Zoology Sample collection Baboon |
| Zdroj: | Journal of Medical Primatology. 41:297-303 |
| ISSN: | 0047-2565 |
| DOI: | 10.1111/j.1600-0684.2012.00555.x |
| Popis: | Background Development of a reproducible baboon in vitro fertilization (IVF) system require optimized and reproducible sperm parameters. The objective of this study was to document basic spermatology values and investigate the reproducibility of these variables in the same baboons 1 or 3 months later in a larger number of baboons. Methods In this prospective study, sperm quality (semen volume, pH, concentration, motility, morphology and size) was evaluated in 27 sperm samples obtained from 9 baboons electroejaculated three times with a time interval of 1 month (between first and second sample collection) and 3 months (between second and third round sample collection). Results Baseline sperm values for semen volume (0.5 ± 0.3 ml), pH (7.5 ± 0.3), concentration (54.2 ± 19.3 million/ml), motility (67.3 ± 18.5%) and morphology (89.2 ± 4.8%) were similar to sperm samples obtained after 1 or 3 months (P > 0.05). Head, midpiece and tail abnormalities were rarely observed (0–9%). Sperm dimensions were characterized by a tail length of 69.6 ± 13.9 μm, a head width of 2.41 ± 0.43 μm and a head length of 3.49 ± 0.6 μm. Conclusion Sperm quality was not affected by repeated electroejaculation with time intervals of 1 or 3 months, suggesting that the same baboon can participate multiple times in reproductive research. |
| Databáze: | OpenAIRE |
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