Application of Sholl analysis to quantify changes in growth and development in rat mammary gland whole mounts
| Autor: | Michael R. Easterling, Suzanne E. Fenton, Jason P. Stanko |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Mammary gland Developmental toxicity Physiology Endocrine Disruptors Biology Toxicology Risk Assessment Article Rats sprague dawley Pattern Recognition Automated Specimen Handling Sholl analysis Rats Sprague-Dawley Mammary Glands Animal Internal medicine Image Interpretation Computer-Assisted medicine Sprague dawley rats Animals Whole mount Sexual Development Age Factors Epithelial Cells Estrogens Rat Mammary Gland Endocrinology medicine.anatomical_structure Estrogen Female Software |
| Zdroj: | Reproductive Toxicology. 54:129-135 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2014.11.004 |
| Popis: | Studies that utilize the rodent mammary gland (MG) as an endpoint for assessing the developmental toxicity of chemical exposures typically employ either basic dimensional measurements or developmental scoring of morphological characteristics as a means to quantify MG development. There are numerous means by which to report these developmental changes, leading to inconsistent translation across laboratories. The Sholl analysis is a method historically used for quantifying neuronal dendritic patterns. The present study describes the use of the Sholl analysis to quantify MG branching characteristics. Using this method, we were able to detect significant differences in branching density in MG of peripubertal female Sprague Dawley rats that had been exposed to vehicle or a potent estrogen. These data suggest the Sholl analysis can be an effective tool for quantitatively measuring an important characteristic of MG development and for examining associations between MG growth and density and adverse effects in the breast. |
| Databáze: | OpenAIRE |
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