Aqueous humor polymerase chain reaction in uveitis – utility and safety

Autor: Argyrios Chronopoulos, Gabriele Thumann, Jorg Dieter Seebach, Daniel Roquelaure, Georges Souteyrand, James Scott Schutz
Rok vydání: 2016
Předmět:
Male
viruses
Eye Infections
Viral
Diagnostic Techniques
Ophthalmological
Ophthalmology & Optometry
Polymerase Chain Reaction
law.invention
0302 clinical medicine
law
hemic and lymphatic diseases
030212 general & internal medicine
Polymerase chain reaction
integumentary system
biology
virus diseases
General Medicine
Middle Aged
Female
Life Sciences & Biomedicine
Toxoplasmosis
Uveitis
Research Article
Adult
medicine.medical_specialty
Congenital cytomegalovirus infection
Aqueous humor
Polymerase chain reaction (PCR)
Virus
Aqueous Humor
Young Adult
03 medical and health sciences
Posterior uveitis
Ophthalmology
parasitic diseases
medicine
Humans
In patient
Aged
Science & Technology
business.industry
Toxoplasma gondii
1113 Ophthalmology And Optometry
medicine.disease
biology.organism_classification
Virology
ddc:616.8
Anterior chamber paracentesis
Anterior uveitis
030221 ophthalmology & optometry
business
Zdroj: BMC Ophthalmology, Vol. 16, No 1 (2016) P. 189
BMC Ophthalmology
ISSN: 1471-2415
Popis: Background: To study the value and safety of aqueous humor polymerase chain reaction (PCR) analysis for Herpes simplex, varicella zoster, cytomegalovirus, Epstein-Barr virus and Toxoplasma gondii in patients with uveitis. Methods: Records of 45 consecutive patients with anterior and posterior uveitis who underwent AC paracentesis with PCR were reviewed. The main outcome measure was frequency of PCR positivity. Secondary outcomes were alteration of treatment, safety of paracentesis, and correlation of keratitic precipitates with PCR positivity, Results: The overall PCR positivity was 48.9 % (22/45). Therapy was changed because of the PCR results in 14/45 patients (37.7 %). One patient experienced a paracentesis related complication (1/45, 2.2 %) without long-term sequelae. Conclusion: Aqueous PCR altered the diagnosis and treatment in over a third of our patients and was relatively safe. Aqueous PCR should be considered for uveitis of atypical clinical appearance, recurrent severe uveitis of uncertain etiology, and therapy refractory cases.
Databáze: OpenAIRE
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