Potential role of the skin and gut microbiota in premenarchal vulvar lichen sclerosus: A pilot case-control study

Autor: Justin D. Arnold, Kalyani Marathe, Amy R. Sapkota, Suhana Chattopadhyay, Lauren E. Hittle, Veronica Gomez-Lobo, Leena Malayil, Emmanuel F. Mongodin
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Prevotella
Physiology
Pilot Projects
Pathogenesis
Plant Science
Gut flora
Peptoniphilus
Pathology and Laboratory Medicine
Pediatrics
Biochemistry
Medicine and Health Sciences
Child
Immune Response
Phylogeny
Skin
Vulvar Lichen Sclerosus
Multidisciplinary
Parvimonas
Biodiversity
Peptostreptococcus
Nucleic acids
Lichen Sclerosus et Atrophicus
Ribosomal RNA
Research Design
Lichenology
Medicine
Dialister
Female
Akkermansia muciniphila
Research Article
Cell biology
food.ingredient
Cellular structures and organelles
Science
Immunology
Biology
Research and Analysis Methods
food
Signs and Symptoms
Humans
Non-coding RNA
Inflammation
Menarche
Bacteria
Gut Bacteria
Organisms
Biology and Life Sciences
Pilot Studies
biology.organism_classification
Gastrointestinal Microbiome
Case-Control Studies
RNA
Clinical Medicine
Ribosomes
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 1, p e0245243 (2021)
ISSN: 1932-6203
Popis: The etiology of vulvar lichen sclerosus (LS) remains unclear; however, alterations in cutaneous and gut microbiota may be contributing to the pathogenesis of this inflammatory condition. To explore this hypothesis, we conducted a pilot case-control study, obtaining dermal swab and stool samples from prepubertal girls with vulvar LS (n = 5), girls with nonspecific vulvovaginitis (n = 5), and healthy controls (n = 3). Samples (n = 56) were subjected to total DNA extractions. Resulting DNA was purified, subjected to PCR (targeting the V3V4 region of the 16S rRNA gene), sequenced, and analyzed using QIIME, MetagenomeSeq, and DESeq2 software packages. Our findings showed that there were significant differences in the cutaneous and gut microbiotas of girls with LS compared to controls. On the skin, girls with LS had a statistically significantly higher relative abundance of Porphyromonas spp., Parvimonas spp., Peptoniphilus spp., Prevotella spp., Dialister spp., and Peptostreptococcus spp., but a lower relative abundance of Cornyebacterium compared to the control group. In the gut samples, girls with LS had a significantly higher relative abundance of Dialister spp., Clostridiales spp., Paraprevotella spp., Escherichia coli, Bifidobacterium adolescentis, and Akkermansia muciniphila, and a lower relative abundance of Roseburia faecis and Ruminococcus bromii compared to controls. These results suggest a potential association between cutaneous and gut dysbiosis and pediatric vulvar LS. Future studies involving larger samples sizes are warranted to further evaluate this association.
Databáze: OpenAIRE
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