Immunization of young heifers with staphylococcal immune evasion proteins before natural exposure to Staphylococcus aureus induces a humoral immune response in serum and milk

Autor: Benedictus, Lindert, Ravesloot, Lars, Poppe, Kim, Daemen, Ineke, Boerhout, Eveline, van Strijp, Jos, Broere, Femke, Rutten, Victor, Koets, Ad, Eisenberg, Susanne, LS GZ Landbouwhuisdieren, Sub Immunologie, LS Immunologie, dI&I RA-I&I I&I, Immunologie
Přispěvatelé: LS GZ Landbouwhuisdieren, Sub Immunologie, LS Immunologie, dI&I RA-I&I I&I, Immunologie
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Cellular immunity
LukM
Milk antibodies
Epidemiology
animal diseases
Mastitis
Natural exposure
medicine.disease_cause
Bacterial/blood
Staphylococcal Infections/immunology
0403 veterinary science
Bacterial Proteins/immunology
Blood serum
Staphylococcal Vaccines/therapeutic use
Humoral/immunology
Medicine
0303 health sciences
lcsh:Veterinary medicine
Milk/immunology
Staphylococcal Vaccines
Antibodies
Bacterial/blood
04 agricultural and veterinary sciences
General Medicine
Staphylococcal Infections
Non-protective immunity
Antibodies
Bacterial
Vaccination
Milk
Staphylococcus aureus
Female
Staphylococcus aureus/immunology
Efb
Research Article
040301 veterinary sciences
Bioinformatica & Diermodellen
Cattle Diseases
chemical and pharmacologic phenomena
Antibodies
03 medical and health sciences
Immune system
Bacterial Proteins
Immunity
Bio-informatics & Animal models
Journal Article
Animals
Epidemiology
Bio-informatics & Animal models
Immune Evasion/immunology
Experimental immunization
Immune Evasion
Epidemiologie
Immunity
Humoral/immunology
Immunization/veterinary
General Veterinary
030306 microbiology
business.industry
Cattle Diseases/immunology
biochemical phenomena
metabolism
and nutrition
veterinary(all)
Immunity
Humoral
Epidemiologie
Bioinformatica & Diermodellen
Humoral immunity
Immunology
Colostrum
bacteria
lcsh:SF600-1100
Immunization
Cattle
business
Zdroj: BMC Veterinary Research, Vol 15, Iss 1, Pp 1-13 (2019)
BMC Veterinary Research 15 (2019) 1
BMC Veterinary Research, 15. BioMed Central
Benedictus, L, Ravesloot, L, Poppe, K, Daemen, I, Boerhout, E, van Strijp, J A G, Broere, F, Rutten, V, Koets, A P & Eisenberg, S 2019, ' Immunization of young heifers with staphylococcal immune evasion proteins before natural exposure to Staphylococcus aureus induces a humoral immune response in serum and milk ', BMC Veterinary Research, vol. 15, no. 1, 15 . https://doi.org/10.1186/s12917-018-1765-9
BMC Veterinary Research, 15(1)
BMC Veterinary Research
BMC Veterinary Research, 15(1). BioMed Central
ISSN: 1746-6148
DOI: 10.1186/s12917-018-1765-9
Popis: Background Staphylococcus aureus, a leading cause of mastitis in dairy cattle, causes severe mastitis and/or chronic persistent infections with detrimental effects on the cows’ wellbeing, lifespan and milk production. Despite years of research there is no effective vaccine against S. aureus mastitis. Boosting of non-protective pre-existing immunity to S. aureus, induced by natural exposure to S. aureus, by vaccination may interfere with vaccine efficacy. The aim was to assess whether experimental immunization of S. aureus naïve animals results in an immune response that differs from immunity following natural exposure to S. aureus. Results First, to define the period during which calves are immunologically naïve for S. aureus, Efb, LukM, and whole-cell S. aureus specific serum antibodies were measured in a cohort of newborn calves by ELISA. Rising S. aureus specific antibodies indicated that from week 12 onward calves mounted an immune response to S. aureus due to natural exposure. Next, an experimental immunization trial was set up using 8-week-old heifer calves (n = 16), half of which were immunized with the immune evasion molecules Efb and LukM. Immunization was repeated after one year and before parturition and humoral and cellular immunity specific for Efb and LukM was determined throughout the study. Post-partum, antibody levels against LukM and EfB were significantly higher in serum, colostrum and milk in the experimentally immunized animals compared to animals naturally exposed to S. aureus. LukM specific IL17a responses were also significantly higher in the immunized cows post-partum. Conclusions Experimental immunization with staphylococcal immune evasion molecules starting before natural exposure resulted in significantly higher antibody levels against Efb and LukM around parturition in serum as well as the site of infection, i.e. in colostrum and milk, compared to natural exposure to S. aureus. This study showed that it is practically feasible to vaccinate S. aureus naïve cattle and that experimental immunization induced a humoral immune response that differed from that after natural exposure only. Electronic supplementary material The online version of this article (10.1186/s12917-018-1765-9) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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