Development and validation of flow cytometry methods for pharmacodynamic clinical biomarkers
| Autor: | Lisa Patti-Diaz, Carla G Hill, Dianna Y. Wu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Biomedical Research
medicine.diagnostic_test Cell growth Clinical Biochemistry Cell General Medicine Validation Studies as Topic Biology Flow Cytometry Bioinformatics Cell counting Analytical Chemistry Flow cytometry Clinical trial Medical Laboratory Technology medicine.anatomical_structure Immunophenotyping medicine Humans Biomarker (medicine) General Pharmacology Toxicology and Pharmaceutics Biomarkers |
| Zdroj: | Bioanalysis. 2:1617-1626 |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio.10.33 |
| Popis: | Flow cytometry is a powerful analytical tool for the analysis of multiple biological parameters of individual cells or particles within heterogeneous cell populations. It has been widely used in biomedical research to perform immunophenotyping, cell counting and numerous cell function assessments, such as intracellular cytokine production, protein phosphorylation, cell proliferation and apoptosis. The implementation of standardized flow cytometry-based biomarker assays in clinical trials remains a challenge due to the limited stability of clinical specimens and the technical variations between instruments. To ensure data quality, it is crucial to develop robust assays for clinical applications. In this review, we summarize current practice in developing, validating and implementing flow cytometry assays to evaluate biomarkers in clinical research. |
| Databáze: | OpenAIRE |
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