Altered expression of the tachykinins substance P/neurokinin A/hemokinin-1 and their preferred neurokinin 1/neurokinin 2 receptors in uterine leiomyomata
Autor: | Francisco M. Pinto, Idaira Dorta, Sara Marrero-Hernández, Delia Báez, Ayoze González-Santana, A. R. Bello, Mariano Hernández, Teresa A. Almeida, Luz Candenas |
---|---|
Přispěvatelé: | Ministerio de Economía y Competitividad (España), European Commission, Gobierno de Canarias |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Adult medicine.medical_specialty animal structures NK1 receptor short isoform Neurokinin A Blotting Western Substance P Biology Real-Time Polymerase Chain Reaction digestive system complex mixtures Leiomyomas 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine TAC1 Internal medicine Tachykinins Tachykinin receptor 1 medicine Biomarkers Tumor Humans RNA Messenger Receptor Uterine leiomyoma Leiomyoma Reverse Transcriptase Polymerase Chain Reaction musculoskeletal neural and ocular physiology Myometrium Obstetrics and Gynecology Receptors Neurokinin-2 Middle Aged Receptors Neurokinin-1 Immunohistochemistry Tachykinin receptors female genital diseases and pregnancy complications Gene Expression Regulation Neoplastic 030104 developmental biology Endocrinology Reproductive Medicine chemistry 030220 oncology & carcinogenesis Uterine Neoplasms Female Tachykinin receptor |
Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
ISSN: | 1556-5653 2011-2556 |
Popis: | [Objective] To study the expression levels of tachykinins and tachykinin receptors in uterine leiomyomas and matched myometrium. [Design] Laboratory study. [Setting] University research laboratories and academic hospital. [Patient(s)] Women undergoing hysterectomy for symptomatic leiomyomas. [Intervention(s)] Quantitative polymerase chain reaction, immunohistochemistry and Western blot. [Main Outcome Measure(s)] Expression and tissue immunostaining of substance P, neurokinin A, hemokinin-1, neurokinin 1 receptor full-length (NK1R-Fl) and truncated (NK1R-Tr) isoforms, and neurokinin 2 receptor (NK2R) in paired samples of leiomyoma and adjacent normal myometrium. [Result(s)] TAC1 messenger RNA (mRNA) was significantly up-regulated in leiomyomas, whereas intense immunoreaction for the three peptides was particularly abundant in connective tissue cells. Differential regulation of TACR1 mRNA was observed, and at the protein level there was a significant increased expression of NK1R short isoform (NK1R-Tr). TACR2 mRNA was significantly up-regulated in leiomyomas, although levels of NK2R protein were similar in normal and tumor cells. [Conclusion(s)] These and our previous data demonstrate that the whole tachykinin system is differentially regulated in leiomyomas. The increased expression of NK1R-Tr might stimulate leiomyoma growth in a similar way to that observed in other steroid-dependent tumors. Supported in part by research grants from the Agencia Canaria de Innovación y Sociedad de la Información, ACIISI, (PI 2007/001), and from Ministerio de Economía y Competitividad (CTQ2011-25564 and RTC-2014-1431-1), Spain, in both cases with joint financing by FEDER funds from the European Union. |
Databáze: | OpenAIRE |
Externí odkaz: |