Altered expression of the tachykinins substance P/neurokinin A/hemokinin-1 and their preferred neurokinin 1/neurokinin 2 receptors in uterine leiomyomata

Autor: Francisco M. Pinto, Idaira Dorta, Sara Marrero-Hernández, Delia Báez, Ayoze González-Santana, A. R. Bello, Mariano Hernández, Teresa A. Almeida, Luz Candenas
Přispěvatelé: Ministerio de Economía y Competitividad (España), European Commission, Gobierno de Canarias
Rok vydání: 2016
Předmět:
0301 basic medicine
Adult
medicine.medical_specialty
animal structures
NK1 receptor short isoform
Neurokinin A
Blotting
Western
Substance P
Biology
Real-Time Polymerase Chain Reaction
digestive system
complex mixtures
Leiomyomas
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
TAC1
Internal medicine
Tachykinins
Tachykinin receptor 1
medicine
Biomarkers
Tumor
Humans
RNA
Messenger
Receptor
Uterine leiomyoma
Leiomyoma
Reverse Transcriptase Polymerase Chain Reaction
musculoskeletal
neural
and ocular physiology
Myometrium
Obstetrics and Gynecology
Receptors
Neurokinin-2
Middle Aged
Receptors
Neurokinin-1
Immunohistochemistry
Tachykinin receptors
female genital diseases and pregnancy complications
Gene Expression Regulation
Neoplastic
030104 developmental biology
Endocrinology
Reproductive Medicine
chemistry
030220 oncology & carcinogenesis
Uterine Neoplasms
Female
Tachykinin receptor
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1556-5653
2011-2556
Popis: [Objective] To study the expression levels of tachykinins and tachykinin receptors in uterine leiomyomas and matched myometrium.
[Design] Laboratory study.
[Setting] University research laboratories and academic hospital.
[Patient(s)] Women undergoing hysterectomy for symptomatic leiomyomas.
[Intervention(s)] Quantitative polymerase chain reaction, immunohistochemistry and Western blot.
[Main Outcome Measure(s)] Expression and tissue immunostaining of substance P, neurokinin A, hemokinin-1, neurokinin 1 receptor full-length (NK1R-Fl) and truncated (NK1R-Tr) isoforms, and neurokinin 2 receptor (NK2R) in paired samples of leiomyoma and adjacent normal myometrium.
[Result(s)] TAC1 messenger RNA (mRNA) was significantly up-regulated in leiomyomas, whereas intense immunoreaction for the three peptides was particularly abundant in connective tissue cells. Differential regulation of TACR1 mRNA was observed, and at the protein level there was a significant increased expression of NK1R short isoform (NK1R-Tr). TACR2 mRNA was significantly up-regulated in leiomyomas, although levels of NK2R protein were similar in normal and tumor cells.
[Conclusion(s)] These and our previous data demonstrate that the whole tachykinin system is differentially regulated in leiomyomas. The increased expression of NK1R-Tr might stimulate leiomyoma growth in a similar way to that observed in other steroid-dependent tumors.
Supported in part by research grants from the Agencia Canaria de Innovación y Sociedad de la Información, ACIISI, (PI 2007/001), and from Ministerio de Economía y Competitividad (CTQ2011-25564 and RTC-2014-1431-1), Spain, in both cases with joint financing by FEDER funds from the European Union.
Databáze: OpenAIRE
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