Frequency of SNP -336A/G in the promoter region of CD209 in a population from northeastern Brazil
| Autor: | Pereira Ac, P.N. Costa, Giovanny R. Pinto, de Oliveira Js, Ferreira Gp, Hygor Ferreira-Fernandes |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Genotype Population Receptors Cell Surface Biology Polymorphism Single Nucleotide law.invention Gene Frequency law Genetics Humans SNP Lectins C-Type Allele Promoter Regions Genetic education Molecular Biology Allele frequency Alleles Polymerase chain reaction Aged Aged 80 and over education.field_of_study Promoter General Medicine Middle Aged Molecular biology Genotype frequency Genetics Population Female Cell Adhesion Molecules Brazil |
| Zdroj: | Genetics and Molecular Research. 14:9395-9403 |
| ISSN: | 1676-5680 |
| Popis: | Dendritic cells (DCs) mediate the initiation of the immune response against a variety of pathogens. The DC-SIGN receptor is encoded by the gene CD209 and is expressed on the surface of DCs. It binds to mannose-rich carbohydrates and enables the recognition of bacteria, fungi, parasites, and viruses. SNP -336A/G in the promoter region of CD209 influences the expression of the DC-SIGN receptor. Several studies have associated this SNP with an increased susceptibility to infectious diseases and the development of more severe forms of disease. Therefore, the aim of this study was to determine the prevalence of SNP -336A/G in a population from northeastern Brazil. We analyzed 181 individuals from the general population of Parnaíba, Piauí, Brazil, of which 37% were men and 63% were women. SNP -336A/G was detected by polymerase chain reaction and treatment with the restriction enzyme MscI and visualized by electrophoresis on an 8% polyacrylamide gel stained with silver nitrate. Of the individuals analyzed, 116 (64.1%) were homozygous AA, 57 (31.5%) were heterozygous (AG), and 8 (4.4%) were homozygous GG. The allele frequency of -336G was 20.2%. Genotype frequencies were in Hardy-Weinberg equilibrium. To the best of our knowledge, this is the first report to describe the frequency of the CD209 SNP -336A/G in a population in the State of Piauí. Further studies are needed to determine the relationship between this SNP and the vulnerability of this population to major infectious diseases. |
| Databáze: | OpenAIRE |
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