Experimental paracoccidioidomycosis in high and low antibody responder mice of Selection IV-A
| Autor: | M. T. Rezkallah-Iwasso, Albano Soares, M. T. S. Peraçoli, M. R. Montenegro, Silvio Luis de Oliveira, C. C. Musatti |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Pathology medicine.medical_specialty Spleen Lesion chemistry.chemical_compound Mice Antigen Corticosterone Antibody Specificity Adrenal Glands medicine Animals Lung Antibodies Fungal Paracoccidioides brasiliensis biology Paracoccidioidomycosis virus diseases General Medicine medicine.disease biology.organism_classification Molecular biology Disease Models Animal Infectious Diseases medicine.anatomical_structure chemistry Liver Granuloma biology.protein Disease Susceptibility medicine.symptom Antibody |
| Zdroj: | Medical mycology. 38(4) |
| ISSN: | 1369-3786 |
| Popis: | High (H) and low (L) responder mice were selected for their ability to produce antibodies against sheep and human erythrocytes (Selection IV-A). In this selection, the difference in antibody responsiveness between H and L lines (HIV-A and LIV-A mice, respectively) was shown to depend mainly on macrophage function. The more rapid catabolism of antigens by macrophages in L mice has been suggested as the main cause of the low antibody production. Due to this high macrophage activity, L animals have been described as more resistant than H animals to intracellular pathogens. These animals were utilized as an experimental model of paracoccidioidomycosis. HIV-A and LIV-A mice were infected with Paracoccidioides brasiliensis by the intravenous route. As expected, H mice were more susceptible to P. brasiliensis with a shorter survival time and higher levels of specific antibodies when compared to L mice. Contrasting with the survival time, the lungs, spleen and liver from H mice showed typical nodular granulomas containing epithelioid and giant cells and few fungi. On the other hand, in LIV-A mice, the lesions of these organs were characterized by looser granulomas with irregular borders and the presence of a large number of fungi. However, the adrenal gland showed different lesion patterns. In H mice these lesions were extensive and characterized by loose granulomas with numerous fungi, while in LIV-A mice the lesions were small and limited to the cortex. Moreover the HIV-A mice presented higher levels of serum corticosterone when compared to LIV-A ones. The higher susceptibility of H mice could be attributed to the extensive lesions of the adrenal glands. These results suggest the use of the H line from the IV-A Selection as an experimental model for further studies of adrenal involvement in paracoccidioidomycosis. |
| Databáze: | OpenAIRE |
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